A fast and reliable method for GHB quantitation in whole blood by GC-MS/MS (TQD) for forensic purposes

André L. Castro; Sónia Tarelho; Mário Dias; Flávio Reis; Helena M. Teixeira

doi:10.1016/j.jpba.2015.11.038

A fast and reliable method for GHB quantitation in whole blood by GC-MS/MS (TQD) for forensic purposes

André L. Castro, Sónia Tarelho, Mário Dias, Flávio Reis, Helena M. Teixeira

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Gamma-hydroxybutyric acid (GHB) is an endogenous compound with a story of clinical use since the 1960s. However, due to its secondary effects, it has become a controlled substance, entering the illicit market. A fully validated, sensitive and reproducible method for the quantification of GHB by methanolic precipitation and GC-MS/MS (TQD) in whole blood is presented. Using 100μL of whole blood, obtained results included a LOD and LLOQ of 0.1mg/L and a recovery of 86% in a working range between 0.1 and 100mg/L. This method is sensitive and specific to detect the presence of GHB in small amounts of whole blood (both ante-mortem or post-mortem), and is, to the authors' knowledge, the first GC-MS-MS TQD method that uses different precursor ions and product ions for the identification of GHB and GHB-D₆ (internal standard). Hence, this method may be especially useful for the study of endogenous values in this biological sample.

Original language	English
Pages (from-to)	139-144
Number of pages	6
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	119
DOIs	https://doi.org/10.1016/j.jpba.2015.11.038
Publication status	Published - 5 Feb 2016
Externally published	Yes

Keywords

Analytical validation
Endogenous values
Forensic science
Forensic toxicology
GC-MS/MS
Gamma-hydroxybutyric acid (GHB)

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10.1016/j.jpba.2015.11.038

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title = "A fast and reliable method for GHB quantitation in whole blood by GC-MS/MS (TQD) for forensic purposes",

abstract = "Gamma-hydroxybutyric acid (GHB) is an endogenous compound with a story of clinical use since the 1960s. However, due to its secondary effects, it has become a controlled substance, entering the illicit market. A fully validated, sensitive and reproducible method for the quantification of GHB by methanolic precipitation and GC-MS/MS (TQD) in whole blood is presented. Using 100μL of whole blood, obtained results included a LOD and LLOQ of 0.1mg/L and a recovery of 86% in a working range between 0.1 and 100mg/L. This method is sensitive and specific to detect the presence of GHB in small amounts of whole blood (both ante-mortem or post-mortem), and is, to the authors' knowledge, the first GC-MS-MS TQD method that uses different precursor ions and product ions for the identification of GHB and GHB-D6 (internal standard). Hence, this method may be especially useful for the study of endogenous values in this biological sample.",

keywords = "Analytical validation, Endogenous values, Forensic science, Forensic toxicology, GC-MS/MS, Gamma-hydroxybutyric acid (GHB)",

author = "Castro, {Andr{\'e} L.} and S{\'o}nia Tarelho and M{\'a}rio Dias and Fl{\'a}vio Reis and Teixeira, {Helena M.}",

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doi = "10.1016/j.jpba.2015.11.038",

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T1 - A fast and reliable method for GHB quantitation in whole blood by GC-MS/MS (TQD) for forensic purposes

AU - Castro, André L.

AU - Tarelho, Sónia

AU - Dias, Mário

AU - Reis, Flávio

AU - Teixeira, Helena M.

PY - 2016/2/5

Y1 - 2016/2/5

N2 - Gamma-hydroxybutyric acid (GHB) is an endogenous compound with a story of clinical use since the 1960s. However, due to its secondary effects, it has become a controlled substance, entering the illicit market. A fully validated, sensitive and reproducible method for the quantification of GHB by methanolic precipitation and GC-MS/MS (TQD) in whole blood is presented. Using 100μL of whole blood, obtained results included a LOD and LLOQ of 0.1mg/L and a recovery of 86% in a working range between 0.1 and 100mg/L. This method is sensitive and specific to detect the presence of GHB in small amounts of whole blood (both ante-mortem or post-mortem), and is, to the authors' knowledge, the first GC-MS-MS TQD method that uses different precursor ions and product ions for the identification of GHB and GHB-D6 (internal standard). Hence, this method may be especially useful for the study of endogenous values in this biological sample.

AB - Gamma-hydroxybutyric acid (GHB) is an endogenous compound with a story of clinical use since the 1960s. However, due to its secondary effects, it has become a controlled substance, entering the illicit market. A fully validated, sensitive and reproducible method for the quantification of GHB by methanolic precipitation and GC-MS/MS (TQD) in whole blood is presented. Using 100μL of whole blood, obtained results included a LOD and LLOQ of 0.1mg/L and a recovery of 86% in a working range between 0.1 and 100mg/L. This method is sensitive and specific to detect the presence of GHB in small amounts of whole blood (both ante-mortem or post-mortem), and is, to the authors' knowledge, the first GC-MS-MS TQD method that uses different precursor ions and product ions for the identification of GHB and GHB-D6 (internal standard). Hence, this method may be especially useful for the study of endogenous values in this biological sample.

KW - Analytical validation

KW - Endogenous values

KW - Forensic science

KW - Forensic toxicology

KW - GC-MS/MS

KW - Gamma-hydroxybutyric acid (GHB)

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SN - 0731-7085

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JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

ER -

A fast and reliable method for GHB quantitation in whole blood by GC-MS/MS (TQD) for forensic purposes

Abstract

Keywords

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