TY - JOUR
T1 - A fast and reliable method for GHB quantitation in whole blood by GC-MS/MS (TQD) for forensic purposes
AU - Castro, André L.
AU - Tarelho, Sónia
AU - Dias, Mário
AU - Reis, Flávio
AU - Teixeira, Helena M.
N1 - Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2016/2/5
Y1 - 2016/2/5
N2 - Gamma-hydroxybutyric acid (GHB) is an endogenous compound with a story of clinical use since the 1960s. However, due to its secondary effects, it has become a controlled substance, entering the illicit market. A fully validated, sensitive and reproducible method for the quantification of GHB by methanolic precipitation and GC-MS/MS (TQD) in whole blood is presented. Using 100μL of whole blood, obtained results included a LOD and LLOQ of 0.1mg/L and a recovery of 86% in a working range between 0.1 and 100mg/L. This method is sensitive and specific to detect the presence of GHB in small amounts of whole blood (both ante-mortem or post-mortem), and is, to the authors' knowledge, the first GC-MS-MS TQD method that uses different precursor ions and product ions for the identification of GHB and GHB-D6 (internal standard). Hence, this method may be especially useful for the study of endogenous values in this biological sample.
AB - Gamma-hydroxybutyric acid (GHB) is an endogenous compound with a story of clinical use since the 1960s. However, due to its secondary effects, it has become a controlled substance, entering the illicit market. A fully validated, sensitive and reproducible method for the quantification of GHB by methanolic precipitation and GC-MS/MS (TQD) in whole blood is presented. Using 100μL of whole blood, obtained results included a LOD and LLOQ of 0.1mg/L and a recovery of 86% in a working range between 0.1 and 100mg/L. This method is sensitive and specific to detect the presence of GHB in small amounts of whole blood (both ante-mortem or post-mortem), and is, to the authors' knowledge, the first GC-MS-MS TQD method that uses different precursor ions and product ions for the identification of GHB and GHB-D6 (internal standard). Hence, this method may be especially useful for the study of endogenous values in this biological sample.
KW - Analytical validation
KW - Endogenous values
KW - Forensic science
KW - Forensic toxicology
KW - GC-MS/MS
KW - Gamma-hydroxybutyric acid (GHB)
UR - http://www.scopus.com/inward/record.url?scp=84949507630&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2015.11.038
DO - 10.1016/j.jpba.2015.11.038
M3 - Article
C2 - 26678181
AN - SCOPUS:84949507630
SN - 0731-7085
VL - 119
SP - 139
EP - 144
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
ER -