TY - JOUR
T1 - Acquisition of flocculation phenotype by Kluyveromyces marxianus when overexpressing GAP1 gene encoding an isoform of glyceraldehyde-3-phosphate dehydrogenase
AU - Almeida, Catarina
AU - Queirós, Odília
AU - Wheals, Alan
AU - Teixeira, José
AU - Moradas-Ferreira, Pedro
N1 - Funding Information:
Catarina Almeida thanks Fundação para a Ciência e Tecnologia (FCT) for providing the grant BD/18203/98.
PY - 2003/11/1
Y1 - 2003/11/1
N2 - The use of flocculating yeast strains has been considered as a convenient approach to obtain high cell densities in bioreactors with increasing productivity in continuous operations. In Kluyveromyces marxianus ATTC 10022, the GAP1 gene encodes an isoform of glyceraldehyde-3-phosphate dehydrogenase-p37 - that is accumulated in the cell wall and is involved in flocculation. To test the use of p37 as a tool for engineering Kluyveromyces cells to display a flocculation phenotype, K. marxianus CCT 3172 was transformed with an expression vector containing GAP1. This vector is based on the pY37 previously described, harbouring a S11 Kluyveromyces origin of replication, and the expression of GAP1 is under the control of GAL1. Kluyveromyces cells overexpressing GAP1 acquired a flocculent phenotype together with the accumulation of p37 in the cell wall. The results support the use of GAP1 gene as a molecular tool for inducing flocculation.
AB - The use of flocculating yeast strains has been considered as a convenient approach to obtain high cell densities in bioreactors with increasing productivity in continuous operations. In Kluyveromyces marxianus ATTC 10022, the GAP1 gene encodes an isoform of glyceraldehyde-3-phosphate dehydrogenase-p37 - that is accumulated in the cell wall and is involved in flocculation. To test the use of p37 as a tool for engineering Kluyveromyces cells to display a flocculation phenotype, K. marxianus CCT 3172 was transformed with an expression vector containing GAP1. This vector is based on the pY37 previously described, harbouring a S11 Kluyveromyces origin of replication, and the expression of GAP1 is under the control of GAL1. Kluyveromyces cells overexpressing GAP1 acquired a flocculent phenotype together with the accumulation of p37 in the cell wall. The results support the use of GAP1 gene as a molecular tool for inducing flocculation.
KW - Flocculation
KW - Glyceraldehyde-3-phosphate dehydrogenase
KW - Kluyveromyces
UR - http://www.scopus.com/inward/record.url?scp=0141643380&partnerID=8YFLogxK
U2 - 10.1016/S0167-7012(03)00189-1
DO - 10.1016/S0167-7012(03)00189-1
M3 - Article
C2 - 14529965
AN - SCOPUS:0141643380
SN - 0167-7012
VL - 55
SP - 433
EP - 440
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
IS - 2
ER -