An efficient poly(pyrrole-viologen)-nitrite reductase biosensor for the mediated detection of nitrite

Serge Da Silva; Serge Cosnier; M. Gabriela Almeida; José J.G. Moura

doi:10.1016/j.elecom.2004.02.007

An efficient poly(pyrrole-viologen)-nitrite reductase biosensor for the mediated detection of nitrite

Serge Da Silva, Serge Cosnier, M. Gabriela Almeida, José J.G. Moura

Research output: Contribution to journal › Article › peer-review

61 Citations (Scopus)

Abstract

A biosensor for nitrite analytical determination was developed using a cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 immobilized and electrically connected on a glassy carbon electrode by entrapment in an electrogenerated poly(pyrrole-viologen) matrix. The modified bioelectrode was studied by cyclic voltammetry and a catalytic current was observed in presence of nitrite. The linear range of the electrode response was 5.4-43.4 μM. The detection limit and the sensitivity were 5.4 μM and 1721 mA M^-1 cm^-2, respectively. The K_M ^app value determined from the Lineweaver-Burk plot was 86 μM. The biosensor fully maintained its electroenzymatic activity towards nitrite after four days. No catalytic response was observed in the presence of nitrate ions while interference from sulfites was considered negligible. Finally, the biosensor composition was optimized in term of monomer-enzyme ratio.

Original language	English
Pages (from-to)	404-408
Number of pages	5
Journal	Electrochemistry Communications
Volume	6
Issue number	4
DOIs	https://doi.org/10.1016/j.elecom.2004.02.007
Publication status	Published - Apr 2004
Externally published	Yes

Keywords

Biosensor
Enzyme wiring
Nitrite reductase
Polypyrrole
Viologen

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10.1016/j.elecom.2004.02.007

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title = "An efficient poly(pyrrole-viologen)-nitrite reductase biosensor for the mediated detection of nitrite",

abstract = "A biosensor for nitrite analytical determination was developed using a cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 immobilized and electrically connected on a glassy carbon electrode by entrapment in an electrogenerated poly(pyrrole-viologen) matrix. The modified bioelectrode was studied by cyclic voltammetry and a catalytic current was observed in presence of nitrite. The linear range of the electrode response was 5.4-43.4 μM. The detection limit and the sensitivity were 5.4 μM and 1721 mA M-1 cm-2, respectively. The KM app value determined from the Lineweaver-Burk plot was 86 μM. The biosensor fully maintained its electroenzymatic activity towards nitrite after four days. No catalytic response was observed in the presence of nitrate ions while interference from sulfites was considered negligible. Finally, the biosensor composition was optimized in term of monomer-enzyme ratio.",

keywords = "Biosensor, Enzyme wiring, Nitrite reductase, Polypyrrole, Viologen",

author = "{Da Silva}, Serge and Serge Cosnier and Almeida, {M. Gabriela} and Moura, {Jos{\'e} J.G.}",

note = "Funding Information: The authors thank the Commission of the European Communities Research Directorate for their support under the PEBCAT project contract No. EVK1-CT-2000-00069, FSE and the Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia, through the PhD Grant No. PRAXIS XXI/BD/11349/97 (MGA)",

year = "2004",

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doi = "10.1016/j.elecom.2004.02.007",

language = "English",

volume = "6",

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T1 - An efficient poly(pyrrole-viologen)-nitrite reductase biosensor for the mediated detection of nitrite

AU - Da Silva, Serge

AU - Cosnier, Serge

AU - Almeida, M. Gabriela

AU - Moura, José J.G.

N1 - Funding Information: The authors thank the Commission of the European Communities Research Directorate for their support under the PEBCAT project contract No. EVK1-CT-2000-00069, FSE and the Fundação para a Ciência e Tecnologia, through the PhD Grant No. PRAXIS XXI/BD/11349/97 (MGA)

PY - 2004/4

Y1 - 2004/4

N2 - A biosensor for nitrite analytical determination was developed using a cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 immobilized and electrically connected on a glassy carbon electrode by entrapment in an electrogenerated poly(pyrrole-viologen) matrix. The modified bioelectrode was studied by cyclic voltammetry and a catalytic current was observed in presence of nitrite. The linear range of the electrode response was 5.4-43.4 μM. The detection limit and the sensitivity were 5.4 μM and 1721 mA M-1 cm-2, respectively. The KM app value determined from the Lineweaver-Burk plot was 86 μM. The biosensor fully maintained its electroenzymatic activity towards nitrite after four days. No catalytic response was observed in the presence of nitrate ions while interference from sulfites was considered negligible. Finally, the biosensor composition was optimized in term of monomer-enzyme ratio.

AB - A biosensor for nitrite analytical determination was developed using a cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 immobilized and electrically connected on a glassy carbon electrode by entrapment in an electrogenerated poly(pyrrole-viologen) matrix. The modified bioelectrode was studied by cyclic voltammetry and a catalytic current was observed in presence of nitrite. The linear range of the electrode response was 5.4-43.4 μM. The detection limit and the sensitivity were 5.4 μM and 1721 mA M-1 cm-2, respectively. The KM app value determined from the Lineweaver-Burk plot was 86 μM. The biosensor fully maintained its electroenzymatic activity towards nitrite after four days. No catalytic response was observed in the presence of nitrate ions while interference from sulfites was considered negligible. Finally, the biosensor composition was optimized in term of monomer-enzyme ratio.

KW - Biosensor

KW - Enzyme wiring

KW - Nitrite reductase

KW - Polypyrrole

KW - Viologen

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DO - 10.1016/j.elecom.2004.02.007

M3 - Article

AN - SCOPUS:1642359212

SN - 1388-2481

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SP - 404

EP - 408

JO - Electrochemistry Communications

JF - Electrochemistry Communications

IS - 4

ER -

An efficient poly(pyrrole-viologen)-nitrite reductase biosensor for the mediated detection of nitrite

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Keywords

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