TY - JOUR
T1 - Application of lactate amperometric sol-gel biosensor to sequential injection determination of l-lactate
AU - Gomes, Sofia Piedade
AU - Odložilíková, Martina
AU - Gabriela Almeida, M.
AU - Araújo, Alberto N.
AU - Couto, Cristina M.C.M.
AU - Montenegro, M. Conceição B.S.M.
N1 - Funding Information:
The authors gratefully acknowledge Fundação para a Ciência e Tecnologia (Projects Praxis/P/QUI/10109 and POCI/QUI/58026/2004) and REQUIMTE for the financial support. S.A.L.P. Gomes thanks Ph.D. Grant (SFRH/BD/13653/2003).
PY - 2007/3/12
Y1 - 2007/3/12
N2 - This work describes the construction and evaluation of lactate sol-gel biosensors to accomplish the determination of lactate in pharmaceutical products. Lactate oxidase was incorporated in a porous sol-gel film placed onto a platinum-based electrode. Acid and basic catalysis were assessed. When coupled to a sequential injection system (SIA) the biosensor, based on (3-aminopropyl)trimethoxysilane, 2-(3,4-epoxycyclohexyl)ethyl-trimethoxysilane, deionised water, polyethylene glycol 6000 and acid catalyst, presented a range of linearity of 5 × 10-5 to 5 × 10-3 M. The analytical usefulness of the developed biosensor was evaluated through analysis of commercial pharmaceutical products containing lactate with a sampling rate of 40 samples h-1. The enzyme remained active for at least 30 days, enabling about 700 determinations without sensitivity decrease.
AB - This work describes the construction and evaluation of lactate sol-gel biosensors to accomplish the determination of lactate in pharmaceutical products. Lactate oxidase was incorporated in a porous sol-gel film placed onto a platinum-based electrode. Acid and basic catalysis were assessed. When coupled to a sequential injection system (SIA) the biosensor, based on (3-aminopropyl)trimethoxysilane, 2-(3,4-epoxycyclohexyl)ethyl-trimethoxysilane, deionised water, polyethylene glycol 6000 and acid catalyst, presented a range of linearity of 5 × 10-5 to 5 × 10-3 M. The analytical usefulness of the developed biosensor was evaluated through analysis of commercial pharmaceutical products containing lactate with a sampling rate of 40 samples h-1. The enzyme remained active for at least 30 days, enabling about 700 determinations without sensitivity decrease.
KW - LOx amperometric biosensor
KW - Pharmaceutical products
KW - SIA
KW - Sol-gel
UR - http://www.scopus.com/inward/record.url?scp=33847186319&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2006.11.027
DO - 10.1016/j.jpba.2006.11.027
M3 - Article
C2 - 17207600
AN - SCOPUS:33847186319
SN - 0731-7085
VL - 43
SP - 1376
EP - 1381
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
IS - 4
ER -