TY - JOUR
T1 - Association between periodontitis and peripheral markers of innate immunity activation and inflammation
AU - Dopico, José
AU - Botelho, João
AU - Ouro, Alberto
AU - Domínguez, Clara
AU - Machado, Vanessa
AU - Aramburu-Nuñez, Marta
AU - Custodia, Antía
AU - Blanco, Teresa
AU - Vázquez-Reza, María
AU - Romaus-Sanjurjo, Daniel
AU - Blanco, Juan
AU - Leira, Rogelio
AU - Sobrino, Tomás
AU - Leira, Yago
N1 - Funding Information:
This study was partially supported by grants from the Xunta de Galicia (TS: IN607A2018/3, TS: IN607D 2020/09, AC: IN606A‐2021/015; DRS: IN606B‐2021/010), Science Ministry of Spain (TS: RTI2018‐102165‐B‐I00), and Institute of Health Carlos III (RL: PI15/01578). Furthermore, this study was also supported by grants from the INTERREG Atlantic Area (TS: EAPA_791/2018_ NEUROATLANTIC project), INTERREG V A España Portugal (POCTEP) (TS: 0624_2IQBIONEURO_6_E), and the European Regional Development Fund (ERDF). Moreover, MA‐N (IFI18/00008), DRS (CD21/00166), and TS (CPII17/00027) are recipients of research contracts from the i‐PFIS, Sara Borrell, and Miguel Servet Programs, respectively, from the Instituto de Salud Carlos III. YL is supported by a research contract in the context of the INTERREG Atlantic Area European Funding Programme (EAPA_791/2018_ NEUROATLANTIC project). AC is supported by a fellowship from the Xunta de Galicia (IN606A‐2021/015). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher Copyright:
© 2022 American Academy of Periodontology.
PY - 2022
Y1 - 2022
N2 - Background: Immune response leading to increased systemic inflammation is one of the mechanisms linking periodontitis to chronic inflammatory diseases. The aim of this study was to compare the expression of toll-like receptors 2 and 4 in monocytes and neutrophils (TLR2M, TLR2N, TLR4M, and TLR4N) and its endogenous ligands (cellular fibronectin [cFN] and heat shock protein 60 [HSP60]) in patients with and without periodontitis. Additionally, the relationship between cFN and HSP60 expression with innate immunity activation and systemic inflammatory response (interleukin 6 [IL-6]) was also evaluated. Methods: A case-controlled study was designed in which 30 patients with periodontitis (cases) and 30 age- and sex-matched participants without periodontitis (controls) were included. Fasting blood samples were collected to determine: (1) expression of TLR2N, TLR2M, TLR4N, and TLR4M by flow cytometry; and (2) serum concentrations of cFN, HSP60, and IL-6 by ELISA technique. Results: Expression of TLR2M (411.5 [314.2, 460.0] vs. 236.5 [204.0, 333.0] AFU), TLR2N (387.0 [332.0, 545.5] vs 230.0 [166.2, 277.7] AFU), TLR4M (2478.5 [1762.2, 2828.0] vs 1705.0 [1274.5, 1951.2] AFU), and TLR4N (2791.0 [2306.7, 3226.2] vs. 1866.0 [1547.5, 2687.2] AFU) as well as serum levels of cFN (301.1 [222.2, 410.9] vs. 156.4 [115.3, 194.0] ng/ml) and IL-6 (10.4 [6.5, 11.5] vs. 3.5 [2.6, 4.9] pg/ml) were significantly higher in periodontitis patients than those without periodontitis. A positive association was found between periodontitis and cFN (odds ratio [OR] = 1.028, p < 0.001), TLR2N (OR = 1.026, p < 0.001), TLR4M (OR = 1.001, p = 0.002), and IL-6 (OR = 1.774, p < 0.001). Conclusions: Periodontitis patients exhibited high expression of TLRs, cFN, and IL-6.
AB - Background: Immune response leading to increased systemic inflammation is one of the mechanisms linking periodontitis to chronic inflammatory diseases. The aim of this study was to compare the expression of toll-like receptors 2 and 4 in monocytes and neutrophils (TLR2M, TLR2N, TLR4M, and TLR4N) and its endogenous ligands (cellular fibronectin [cFN] and heat shock protein 60 [HSP60]) in patients with and without periodontitis. Additionally, the relationship between cFN and HSP60 expression with innate immunity activation and systemic inflammatory response (interleukin 6 [IL-6]) was also evaluated. Methods: A case-controlled study was designed in which 30 patients with periodontitis (cases) and 30 age- and sex-matched participants without periodontitis (controls) were included. Fasting blood samples were collected to determine: (1) expression of TLR2N, TLR2M, TLR4N, and TLR4M by flow cytometry; and (2) serum concentrations of cFN, HSP60, and IL-6 by ELISA technique. Results: Expression of TLR2M (411.5 [314.2, 460.0] vs. 236.5 [204.0, 333.0] AFU), TLR2N (387.0 [332.0, 545.5] vs 230.0 [166.2, 277.7] AFU), TLR4M (2478.5 [1762.2, 2828.0] vs 1705.0 [1274.5, 1951.2] AFU), and TLR4N (2791.0 [2306.7, 3226.2] vs. 1866.0 [1547.5, 2687.2] AFU) as well as serum levels of cFN (301.1 [222.2, 410.9] vs. 156.4 [115.3, 194.0] ng/ml) and IL-6 (10.4 [6.5, 11.5] vs. 3.5 [2.6, 4.9] pg/ml) were significantly higher in periodontitis patients than those without periodontitis. A positive association was found between periodontitis and cFN (odds ratio [OR] = 1.028, p < 0.001), TLR2N (OR = 1.026, p < 0.001), TLR4M (OR = 1.001, p = 0.002), and IL-6 (OR = 1.774, p < 0.001). Conclusions: Periodontitis patients exhibited high expression of TLRs, cFN, and IL-6.
KW - fibronectin
KW - inflammation
KW - innate immunity
KW - periodontitis
KW - toll-like receptor
UR - http://www.scopus.com/inward/record.url?scp=85134524576&partnerID=8YFLogxK
U2 - 10.1002/JPER.22-0216
DO - 10.1002/JPER.22-0216
M3 - Article
C2 - 35665930
AN - SCOPUS:85134524576
SN - 0022-3492
VL - 94
SP - 11
EP - 19
JO - Journal of Periodontology
JF - Journal of Periodontology
IS - 1
ER -