Besnoitia besnoiti tachyzoites induce monocyte extracellular trap formation

Tamara Muñoz-Caro, Liliana M.R. Silva, Christin Ritter, Anja Taubert, Carlos Hermosilla

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

Extracellular trap (ET) formation has been demonstrated as an important novel effector mechanism of polymorphonuclear neutrophils (PMN), eosinophils, mast cells and macrophages acting extracellularly against pathogens. In the present study, we show that tachyzoites of the emerging apicomplexan parasite Besnoitia besnoiti, that have recently been reported as potent inducers of PMN-derived ETosis, also trigger the release of ETs in an additional cell type, namely in monocytes. Fluorescence illustrations as well as scanning electron microscopy analyses (SEM) showed monocyte-promoted ET formation to be rapidly induced upon exposure to viable tachyzoites of B. besnoiti. Classical characteristics of ETs were confirmed by the co-localization of extracellular DNA with histones (H3) or myeloperoxidase (MPO) in parasite-entrapping structures. Monocyte-derived ETs were efficiently abolished by DNase I treatment and significantly reduced by treatments with inhibitors of MPO and NADPH oxidase, thus strengthening the key roles of reactive oxygen species (ROS) and MPO in monocyte ET formation. For comparative reasons, we additionally tested sporozoite stages of the closely related parasite Eimeria bovis for their capacity to induce monocyte-derived ETs and showed that these stages indeed induce ETs. To our best knowledge, we here report for the first time on monocyte ETs against the apicomplexan parasites B. besnoiti and E. bovis. Our results indicate that monocyte-triggered ETs may represent an important effector mechanism of the host early innate immune response against B. besnoiti and add a new cell type to the list of cells capable to release ETs.

Original languageEnglish
Pages (from-to)4189-4197
Number of pages9
JournalParasitology Research
Volume113
Issue number11
DOIs
Publication statusPublished - 17 Oct 2014
Externally publishedYes

Keywords

  • Besnoitia besnoiti
  • Eimeria bovis
  • Extracellular traps
  • Monocytes

Fingerprint

Dive into the research topics of 'Besnoitia besnoiti tachyzoites induce monocyte extracellular trap formation'. Together they form a unique fingerprint.

Cite this