TY - JOUR
T1 - Blood collection from the external jugular vein of Oryctolagus cuniculus algirus sedated with midazolam
T2 - live sampling of a subspecies at risk
AU - Dos Santos, Fabio A.Abade
AU - Carvalho, Carina L.
AU - Peleteiro, M. Conceição
AU - Gabrie, Sofia Isabel
AU - Patrício, Rui
AU - Carvalho, João
AU - Cunha, Mónica V.
AU - Duarte, Margarida D.
N1 - Publisher Copyright:
© 2019 The Authors.
PY - 2019
Y1 - 2019
N2 - In the last decades, the European wild rabbit, particularly the Oryctolagus cuniculus algirus, a keystone species in the Iberian Peninsula ecosystems, declined severely, raising concerns from the wildlife authorities. The hunting calendar in Portugal limits sampling collection to a narrow window of few months annually. Nevertheless, governmental wildlife protection laws allow live rabbit sampling for population and sanitary evaluations. The aim of this study is to adjust blood collection protocols from the external jugular vein (EJV) described for domestic rabbits to the wild rabbit. Collection of peripheral blood is problematic in the wild rabbit given its small body size and the reduced calibre of vessels but mostly its nervous disposition and fragility. We describe in detail a procedure for EJV blood collection in 30 wild rabbits after sedation with midazolam. Emphasis is given to protocol adjustments for wild rabbit. Heart rate, respiratory rate and body temperature were assessed before sedation, after sedation but before collection and after blood collection. Sedation onset took on mean (SD) 8 ± 2 min. The technique allowed the collection at least 1 ml of blood, a satisfactory volume for routine laboratory testing. The differences observed in heart and respiratory rates before and after blood collection were not statistically significant, indicating that no cardiorespiratory interference occurred due to venepuncture. Recovery from sedation took on mean (SD) 17 ± 2 min. All animals were set free during the first hour after blood collection. This work aims to demonstrate that blood collection under sedation is a safe and feasible procedure in wild rabbits when practiced by experienced veterinarians. At no time, whatsoever, was the physiological homeostasis at risk and no injuries were inflicted on the animals. To our knowledge this report constitutes the first guided description of blood collection from the EJV in sedated O. c. algirus and the first collection of physiological parameters measured under different conditions.
AB - In the last decades, the European wild rabbit, particularly the Oryctolagus cuniculus algirus, a keystone species in the Iberian Peninsula ecosystems, declined severely, raising concerns from the wildlife authorities. The hunting calendar in Portugal limits sampling collection to a narrow window of few months annually. Nevertheless, governmental wildlife protection laws allow live rabbit sampling for population and sanitary evaluations. The aim of this study is to adjust blood collection protocols from the external jugular vein (EJV) described for domestic rabbits to the wild rabbit. Collection of peripheral blood is problematic in the wild rabbit given its small body size and the reduced calibre of vessels but mostly its nervous disposition and fragility. We describe in detail a procedure for EJV blood collection in 30 wild rabbits after sedation with midazolam. Emphasis is given to protocol adjustments for wild rabbit. Heart rate, respiratory rate and body temperature were assessed before sedation, after sedation but before collection and after blood collection. Sedation onset took on mean (SD) 8 ± 2 min. The technique allowed the collection at least 1 ml of blood, a satisfactory volume for routine laboratory testing. The differences observed in heart and respiratory rates before and after blood collection were not statistically significant, indicating that no cardiorespiratory interference occurred due to venepuncture. Recovery from sedation took on mean (SD) 17 ± 2 min. All animals were set free during the first hour after blood collection. This work aims to demonstrate that blood collection under sedation is a safe and feasible procedure in wild rabbits when practiced by experienced veterinarians. At no time, whatsoever, was the physiological homeostasis at risk and no injuries were inflicted on the animals. To our knowledge this report constitutes the first guided description of blood collection from the EJV in sedated O. c. algirus and the first collection of physiological parameters measured under different conditions.
KW - Oryctolagus cuniculus
KW - blood collection
KW - external jugular vein
KW - sampling live animals
KW - sanitary surveillance
KW - venepuncture
KW - wild rabbit
UR - http://www.scopus.com/inward/record.url?scp=85127695163&partnerID=8YFLogxK
U2 - 10.2981/wlb.00588
DO - 10.2981/wlb.00588
M3 - Article
AN - SCOPUS:85127695163
SN - 0909-6396
VL - 2019
JO - Wildlife Biology
JF - Wildlife Biology
IS - 1
M1 - wlb.00588
ER -