TY - JOUR
T1 - Botulinum toxin type A reduces inflammatory hypernociception induced by arthritis in the temporomadibular joint of rats
AU - Lora, Victor Ricardo Manuel Muñoz
AU - Clemente-Napimoga, Juliana Trindade
AU - Abdalla, Henrique Ballassini
AU - Macedo, Cristina Gomes
AU - Canales, Giancarlo de la Torre
AU - Barbosa, Celia Marisa Rizzatti
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Objective This study aimed to investigate the antinociceptive effects of Botulinum toxin type A (BoNT-A) on persistent inflammatory hypernociception induced by arthritis in the temporomandibular joint (TMJ) of rats. Material and methods Wistar rats were induced to persistent inflammatory hypernociception in the left TMJ. Then, animals were treated with intra-TMJ injections of BoNT-A, using doses of 3.5, 7 and 14 U/kg. Saline was used as control group. Behavioral tests were applied to evaluated the effect of BoNT-A in the inflammatory hypernociception. After that, animals were euthanized and samples from peri-articular tissues and trigeminal ganglia were obtained for further analyses. Results BoNT-A reduced the persistent inflammatory hypernociception induced by arthritis in the TMJ of rats. BoNT-A significantly reduced the peripheral release of the neurotransmitters Substance P and Calcitonin gene related peptide; and the pro-inflammatory cytokine IL-1β. Otherwise, BoNT-A had no effect in the peripheral release of glutamate and the cytokine TNF-α. Conclusion These results demonstrate that intra-articular injection of BoNT-A reduces the albumin-induced arthritis persistent hypernociception in TMJ of rats by peripheral inhibition of neuropeptides release.
AB - Objective This study aimed to investigate the antinociceptive effects of Botulinum toxin type A (BoNT-A) on persistent inflammatory hypernociception induced by arthritis in the temporomandibular joint (TMJ) of rats. Material and methods Wistar rats were induced to persistent inflammatory hypernociception in the left TMJ. Then, animals were treated with intra-TMJ injections of BoNT-A, using doses of 3.5, 7 and 14 U/kg. Saline was used as control group. Behavioral tests were applied to evaluated the effect of BoNT-A in the inflammatory hypernociception. After that, animals were euthanized and samples from peri-articular tissues and trigeminal ganglia were obtained for further analyses. Results BoNT-A reduced the persistent inflammatory hypernociception induced by arthritis in the TMJ of rats. BoNT-A significantly reduced the peripheral release of the neurotransmitters Substance P and Calcitonin gene related peptide; and the pro-inflammatory cytokine IL-1β. Otherwise, BoNT-A had no effect in the peripheral release of glutamate and the cytokine TNF-α. Conclusion These results demonstrate that intra-articular injection of BoNT-A reduces the albumin-induced arthritis persistent hypernociception in TMJ of rats by peripheral inhibition of neuropeptides release.
KW - Botulinum toxins type A
KW - Neurotransmitters
KW - Nociception
UR - http://www.scopus.com/inward/record.url?scp=85013129836&partnerID=8YFLogxK
U2 - 10.1016/j.toxicon.2017.02.010
DO - 10.1016/j.toxicon.2017.02.010
M3 - Article
C2 - 28209481
AN - SCOPUS:85013129836
SN - 0041-0101
VL - 129
SP - 52
EP - 57
JO - Toxicon
JF - Toxicon
ER -
