Cloning of two β-tubulin related genes in tetrahymena pyriformis

Isabel Barahona; Lisete Galego; Michel Jacquet; Claudina Rodrigues-Pousada

doi:10.1016/0014-5793(85)80377-X

Cloning of two β-tubulin related genes in tetrahymena pyriformis

Isabel Barahona, Lisete Galego, Michel Jacquet, Claudina Rodrigues-Pousada

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Genomic clones containing β-tubulin sequences were isolated from a pBR322 genomic library of Tetrahymena pyriformis. Each clone has been mapped and the identity of the tubulin-related genes has been established by cross-hybridization with a cDNA containing a complete β-tubulin coding region from Chlamydomonas and by the positive hybrid-selected translation assay. The respective tubulin-related genes, βTT₁ and βTT₂, differ from each other although they contain a homologous region which is also homologous with a heterologous probe. Tetrahymena β-tubulin-related genes are physically linked and clustered in the genome.

Original language	English
Pages (from-to)	227-232
Number of pages	6
Journal	FEBS Letters
Volume	188
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(85)80377-X
Publication status	Published - 2 Sept 1985
Externally published	Yes

Keywords

Gene organization
Tetrahymena pyriformis
β-Tubulin

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10.1016/0014-5793(85)80377-X

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title = "Cloning of two β-tubulin related genes in tetrahymena pyriformis",

abstract = "Genomic clones containing β-tubulin sequences were isolated from a pBR322 genomic library of Tetrahymena pyriformis. Each clone has been mapped and the identity of the tubulin-related genes has been established by cross-hybridization with a cDNA containing a complete β-tubulin coding region from Chlamydomonas and by the positive hybrid-selected translation assay. The respective tubulin-related genes, βTT1 and βTT2, differ from each other although they contain a homologous region which is also homologous with a heterologous probe. Tetrahymena β-tubulin-related genes are physically linked and clustered in the genome.",

keywords = "Gene organization, Tetrahymena pyriformis, β-Tubulin",

author = "Isabel Barahona and Lisete Galego and Michel Jacquet and Claudina Rodrigues-Pousada",

note = "Funding Information: and to Mrs Ana Barreiros for her skilful technical assistance.T his work was supported by grants from the Centre National de la RechercheS cien-tifique and CalousteG ulbenkianF oundation. I.B. thanks the French Governmentf or a fellowship.",

year = "1985",

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day = "2",

doi = "10.1016/0014-5793(85)80377-X",

language = "English",

volume = "188",

pages = "227--232",

journal = "FEBS Letters",

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TY - JOUR

T1 - Cloning of two β-tubulin related genes in tetrahymena pyriformis

AU - Barahona, Isabel

AU - Galego, Lisete

AU - Jacquet, Michel

AU - Rodrigues-Pousada, Claudina

N1 - Funding Information: and to Mrs Ana Barreiros for her skilful technical assistance.T his work was supported by grants from the Centre National de la RechercheS cien-tifique and CalousteG ulbenkianF oundation. I.B. thanks the French Governmentf or a fellowship.

PY - 1985/9/2

Y1 - 1985/9/2

N2 - Genomic clones containing β-tubulin sequences were isolated from a pBR322 genomic library of Tetrahymena pyriformis. Each clone has been mapped and the identity of the tubulin-related genes has been established by cross-hybridization with a cDNA containing a complete β-tubulin coding region from Chlamydomonas and by the positive hybrid-selected translation assay. The respective tubulin-related genes, βTT1 and βTT2, differ from each other although they contain a homologous region which is also homologous with a heterologous probe. Tetrahymena β-tubulin-related genes are physically linked and clustered in the genome.

AB - Genomic clones containing β-tubulin sequences were isolated from a pBR322 genomic library of Tetrahymena pyriformis. Each clone has been mapped and the identity of the tubulin-related genes has been established by cross-hybridization with a cDNA containing a complete β-tubulin coding region from Chlamydomonas and by the positive hybrid-selected translation assay. The respective tubulin-related genes, βTT1 and βTT2, differ from each other although they contain a homologous region which is also homologous with a heterologous probe. Tetrahymena β-tubulin-related genes are physically linked and clustered in the genome.

KW - Gene organization

KW - Tetrahymena pyriformis

KW - β-Tubulin

UR - https://www.scopus.com/pages/publications/0022219693

U2 - 10.1016/0014-5793(85)80377-X

DO - 10.1016/0014-5793(85)80377-X

M3 - Article

AN - SCOPUS:0022219693

SN - 0014-5793

VL - 188

SP - 227

EP - 232

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Cloning of two β-tubulin related genes in tetrahymena pyriformis

Abstract

Keywords

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