Cloning, sequencing and expression in the seeds and radicles of two Lupinus albus conglutin γ genes

Alessio Scarafoni; Annalisa Di Cataldo; Tatiana D. Vassilevskaia; Evguenia P. Bekman; Claudina Rodrigues-Pousada; Fabrizio Ceciliani; Marcello Duranti

doi:10.1016/S0167-4781(01)00225-1

Cloning, sequencing and expression in the seeds and radicles of two Lupinus albus conglutin γ genes

Alessio Scarafoni, Annalisa Di Cataldo, Tatiana D. Vassilevskaia, Evguenia P. Bekman, Claudina Rodrigues-Pousada, Fabrizio Ceciliani, Marcello Duranti

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

Two genes encoding conglutin γ have been isolated from a Lupinus albus genomic library and sequenced. The expression of conglutin γ was studied by partial amino acid sequencing of the mature seed protein and by nucleotide sequencing of reverse transcriptase-polymerase chain reaction products from various tissues during the plant life cycle.

Original language	English
Pages (from-to)	147-151
Number of pages	5
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1519
Issue number	1-2
DOIs	https://doi.org/10.1016/S0167-4781(01)00225-1
Publication status	Published - 28 May 2001
Externally published	Yes

Keywords

Conglutin γ
Germinating seed
Leguminous plant
Lupinus albus
Young root

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10.1016/S0167-4781(01)00225-1

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title = "Cloning, sequencing and expression in the seeds and radicles of two Lupinus albus conglutin γ genes",

abstract = "Two genes encoding conglutin γ have been isolated from a Lupinus albus genomic library and sequenced. The expression of conglutin γ was studied by partial amino acid sequencing of the mature seed protein and by nucleotide sequencing of reverse transcriptase-polymerase chain reaction products from various tissues during the plant life cycle.",

keywords = "Conglutin γ, Germinating seed, Leguminous plant, Lupinus albus, Young root",

author = "Alessio Scarafoni and {Di Cataldo}, Annalisa and Vassilevskaia, {Tatiana D.} and Bekman, {Evguenia P.} and Claudina Rodrigues-Pousada and Fabrizio Ceciliani and Marcello Duranti",

note = "Funding Information: This work was funded by grants MURST 60% (1998/1999 and 1999/2000) and by the grant Praxis/2/2.1/BIO/1146/95 awarded by FCT to C.R.-P.",

year = "2001",

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doi = "10.1016/S0167-4781(01)00225-1",

language = "English",

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pages = "147--151",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

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T1 - Cloning, sequencing and expression in the seeds and radicles of two Lupinus albus conglutin γ genes

AU - Scarafoni, Alessio

AU - Di Cataldo, Annalisa

AU - Vassilevskaia, Tatiana D.

AU - Bekman, Evguenia P.

AU - Rodrigues-Pousada, Claudina

AU - Ceciliani, Fabrizio

AU - Duranti, Marcello

N1 - Funding Information: This work was funded by grants MURST 60% (1998/1999 and 1999/2000) and by the grant Praxis/2/2.1/BIO/1146/95 awarded by FCT to C.R.-P.

PY - 2001/5/28

Y1 - 2001/5/28

N2 - Two genes encoding conglutin γ have been isolated from a Lupinus albus genomic library and sequenced. The expression of conglutin γ was studied by partial amino acid sequencing of the mature seed protein and by nucleotide sequencing of reverse transcriptase-polymerase chain reaction products from various tissues during the plant life cycle.

AB - Two genes encoding conglutin γ have been isolated from a Lupinus albus genomic library and sequenced. The expression of conglutin γ was studied by partial amino acid sequencing of the mature seed protein and by nucleotide sequencing of reverse transcriptase-polymerase chain reaction products from various tissues during the plant life cycle.

KW - Conglutin γ

KW - Germinating seed

KW - Leguminous plant

KW - Lupinus albus

KW - Young root

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DO - 10.1016/S0167-4781(01)00225-1

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AN - SCOPUS:0035962825

SN - 0167-4781

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 1-2

ER -

Cloning, sequencing and expression in the seeds and radicles of two Lupinus albus conglutin γ genes

Abstract

Keywords

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