Construction and characterization of CD4-independent infectious recombinant HIV-2 molecular clones

J. M. Azevedo-Pereira; Q. Santos-Costa; N. Taveira; F. Veríssimo; J. Moniz-Pereira

doi:10.1016/j.virusres.2003.08.008

Construction and characterization of CD4-independent infectious recombinant HIV-2 molecular clones

J. M. Azevedo-Pereira, Q. Santos-Costa, N. Taveira, F. Veríssimo, J. Moniz-Pereira

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) differ in their pathogenic mechanisms as evidenced by lower rate of disease progression, lower transmission rates and lower viral load in peripheral blood for HIV-2. One of the many factors that are involved in these characteristics is the interaction between viral glycoproteins and cellular receptors. The study of these interactions in an HIV-2 model could lead to important conclusions regarding pathogenesis and transmission mechanisms of HIV-2 infection. Here we report the design of a method enabling the construction of recombinant proviral HIV-2 DNAs in a moderate copy number plasmid that allows the analysis of env gene structure and functionality. This method constitutes an important tool for the study of HIV-2 env glycoproteins and for the mappings of genetic determinants of HIV-2 coreceptor usage and CD4-independent interaction. Furthermore, this knowledge will help towards the understanding of the different pathogenic mechanisms of HIV-1 and HIV-2.

Original language	English
Pages (from-to)	159-163
Number of pages	5
Journal	Virus Research
Volume	97
Issue number	2
DOIs	https://doi.org/10.1016/j.virusres.2003.08.008
Publication status	Published - Nov 2003

Keywords

CD4-independent
Envelope glycoprotein
HIV-2
Molecular clone
env gene

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virusres.2003.08.008

Cite this

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title = "Construction and characterization of CD4-independent infectious recombinant HIV-2 molecular clones",

abstract = "Human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) differ in their pathogenic mechanisms as evidenced by lower rate of disease progression, lower transmission rates and lower viral load in peripheral blood for HIV-2. One of the many factors that are involved in these characteristics is the interaction between viral glycoproteins and cellular receptors. The study of these interactions in an HIV-2 model could lead to important conclusions regarding pathogenesis and transmission mechanisms of HIV-2 infection. Here we report the design of a method enabling the construction of recombinant proviral HIV-2 DNAs in a moderate copy number plasmid that allows the analysis of env gene structure and functionality. This method constitutes an important tool for the study of HIV-2 env glycoproteins and for the mappings of genetic determinants of HIV-2 coreceptor usage and CD4-independent interaction. Furthermore, this knowledge will help towards the understanding of the different pathogenic mechanisms of HIV-1 and HIV-2.",

keywords = "CD4-independent, Envelope glycoprotein, HIV-2, Molecular clone, env gene",

author = "Azevedo-Pereira, {J. M.} and Q. Santos-Costa and N. Taveira and F. Ver{\'i}ssimo and J. Moniz-Pereira",

note = "Funding Information: We are grateful to Dr. K. Peden for the kindly gift of pROD10 plasmid. We also wish to acknowledge Drs. Littman and KewalRamani—the Centralised Facility for AIDS Reagents (EU Programme EVA/MRC; contract QLK2-CT-1999-00606) and the UK Medical Research Council for the GHOST cell lines. The following reagents were obtained through the AIDS Research and Reference Program, Division of AIDS, NIAID, NIH: Cf2Th/synCCR5 cell line from Dr. T. Mirzabekov and Dr. J. Sodroski, and CD4 MAb SIM.4 from Dr. J. Hildreth. This work was supported by grants of Junta Nacional de Investiga{\c c}{\~a}o Cient{\'ı}fica (Programa PRAXIS XXI), Comiss{\~a}o Nacional de Luta contra a SIDA and from Funda{\c c}{\~a}o da Ci{\^e}ncia e Tecnologia (FCT grant POCTI/ESP/39987/2001). Q.S.-C. is a recipient of a Ph.D. fellowship from FCT.",

year = "2003",

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doi = "10.1016/j.virusres.2003.08.008",

language = "English",

volume = "97",

pages = "159--163",

journal = "Virus Research",

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T1 - Construction and characterization of CD4-independent infectious recombinant HIV-2 molecular clones

AU - Azevedo-Pereira, J. M.

AU - Santos-Costa, Q.

AU - Taveira, N.

AU - Veríssimo, F.

AU - Moniz-Pereira, J.

N1 - Funding Information: We are grateful to Dr. K. Peden for the kindly gift of pROD10 plasmid. We also wish to acknowledge Drs. Littman and KewalRamani—the Centralised Facility for AIDS Reagents (EU Programme EVA/MRC; contract QLK2-CT-1999-00606) and the UK Medical Research Council for the GHOST cell lines. The following reagents were obtained through the AIDS Research and Reference Program, Division of AIDS, NIAID, NIH: Cf2Th/synCCR5 cell line from Dr. T. Mirzabekov and Dr. J. Sodroski, and CD4 MAb SIM.4 from Dr. J. Hildreth. This work was supported by grants of Junta Nacional de Investigação Cientı́fica (Programa PRAXIS XXI), Comissão Nacional de Luta contra a SIDA and from Fundação da Ciência e Tecnologia (FCT grant POCTI/ESP/39987/2001). Q.S.-C. is a recipient of a Ph.D. fellowship from FCT.

PY - 2003/11

Y1 - 2003/11

N2 - Human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) differ in their pathogenic mechanisms as evidenced by lower rate of disease progression, lower transmission rates and lower viral load in peripheral blood for HIV-2. One of the many factors that are involved in these characteristics is the interaction between viral glycoproteins and cellular receptors. The study of these interactions in an HIV-2 model could lead to important conclusions regarding pathogenesis and transmission mechanisms of HIV-2 infection. Here we report the design of a method enabling the construction of recombinant proviral HIV-2 DNAs in a moderate copy number plasmid that allows the analysis of env gene structure and functionality. This method constitutes an important tool for the study of HIV-2 env glycoproteins and for the mappings of genetic determinants of HIV-2 coreceptor usage and CD4-independent interaction. Furthermore, this knowledge will help towards the understanding of the different pathogenic mechanisms of HIV-1 and HIV-2.

AB - Human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) differ in their pathogenic mechanisms as evidenced by lower rate of disease progression, lower transmission rates and lower viral load in peripheral blood for HIV-2. One of the many factors that are involved in these characteristics is the interaction between viral glycoproteins and cellular receptors. The study of these interactions in an HIV-2 model could lead to important conclusions regarding pathogenesis and transmission mechanisms of HIV-2 infection. Here we report the design of a method enabling the construction of recombinant proviral HIV-2 DNAs in a moderate copy number plasmid that allows the analysis of env gene structure and functionality. This method constitutes an important tool for the study of HIV-2 env glycoproteins and for the mappings of genetic determinants of HIV-2 coreceptor usage and CD4-independent interaction. Furthermore, this knowledge will help towards the understanding of the different pathogenic mechanisms of HIV-1 and HIV-2.

KW - CD4-independent

KW - Envelope glycoprotein

KW - HIV-2

KW - Molecular clone

KW - env gene

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DO - 10.1016/j.virusres.2003.08.008

M3 - Article

C2 - 14602209

AN - SCOPUS:0242266539

SN - 0168-1702

VL - 97

SP - 159

EP - 163

JO - Virus Research

JF - Virus Research

IS - 2

ER -

Construction and characterization of CD4-independent infectious recombinant HIV-2 molecular clones

Abstract

Keywords

UN SDGs

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