Copper tolerance in marinobacter hydrocarbonoclasticus — proteomic analysis of the periplasm

Célia M. Silveira, Manolis Matzapetakis, M. Gabriela Almeida, Sofia R. Pauleta

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Marinobacter hydrocarbonoclasticus was shown to have a high tolerance to copper sulphate. A minimum inhibitory concentration of 1.6 mM was determined, thus classifying Marinobacter hydrocarbonoclasticus as copper resistant. A proteomic approach based on two-dimensional gel electrophoresis provided a preliminary analysis over the differential protein expression induced by 1 mM of copper in Marinobacter hydrocarbonoclasticus. The copper stress led to a global change in the proteomic profile of the periplasmic fraction of this bacterium, involving modulation of the expression of 53 out of the close to 500 proteins detected, when compared with control growths with trace copper concentration.

Original languageEnglish
Title of host publicationMicrobes in Applied Research
Subtitle of host publicationCurrent Advances and Challenges, Malaga, Spain, 14 - 16 September 2011
PublisherWorld Scientific Publishing Co.
Pages608-612
Number of pages5
ISBN (Electronic)9789814405041
ISBN (Print)9814405035, 9789814405034
DOIs
Publication statusPublished - 1 Jan 2012
Externally publishedYes

Keywords

  • Copper tolerance
  • Marinobacter hydrocarbonoclasticus
  • Proteomics

Fingerprint

Dive into the research topics of 'Copper tolerance in marinobacter hydrocarbonoclasticus — proteomic analysis of the periplasm'. Together they form a unique fingerprint.

Cite this