Detection and quantification of hCG and its isoforms with MALDI-ToF mass spectrometry

Raminta Zmuidinaite; Ray K. Iles; Ricardo J. Pais; Stephen A. Butler

doi:10.1016/B978-0-12-820050-6.00008-4

Detection and quantification of hCG and its isoforms with MALDI-ToF mass spectrometry

Raminta Zmuidinaite, Ray K. Iles, Ricardo J. Pais, Stephen A. Butler

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Citation (Scopus)

Abstract

Primarily, the purpose of hCG detection has been the confirmation of pregnancy by analysis of urine or blood. However, the number of applications for which hCG or hCGβ has been used for diagnostic purposes has significantly grown over the years. Examples of such applications are the detection of hydatidiform mole, cancer, ectopic pregnancy, and fetal trisomies. The wide range of applications renders the accurate and precise detection and quantification of the molecule in various biological fluids necessary. Therefore, in this chapter, the quantification and detection of hCG and its variants using MALDI-ToF mass spectrometry will be discussed.

Original language	English
Title of host publication	100 Years of Human Chorionic Gonadotropin
Subtitle of host publication	Reviews and New Perspectives
Publisher	Elsevier
Pages	75-85
Number of pages	11
ISBN (Electronic)	9780128200506
ISBN (Print)	9780128216095
DOIs	https://doi.org/10.1016/B978-0-12-820050-6.00008-4
Publication status	Published - 1 Jan 2020
Externally published	Yes

Keywords

Detection
Immunoassay
MALDI-ToF MS
Quantitation
hCG isoforms
hCGα
hCGβ
hCGβ core fragment

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/B978-0-12-820050-6.00008-4

Cite this

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title = "Detection and quantification of hCG and its isoforms with MALDI-ToF mass spectrometry",

abstract = "Primarily, the purpose of hCG detection has been the confirmation of pregnancy by analysis of urine or blood. However, the number of applications for which hCG or hCGβ has been used for diagnostic purposes has significantly grown over the years. Examples of such applications are the detection of hydatidiform mole, cancer, ectopic pregnancy, and fetal trisomies. The wide range of applications renders the accurate and precise detection and quantification of the molecule in various biological fluids necessary. Therefore, in this chapter, the quantification and detection of hCG and its variants using MALDI-ToF mass spectrometry will be discussed.",

keywords = "Detection, Immunoassay, MALDI-ToF MS, Quantitation, hCG isoforms, hCGα, hCGβ, hCGβ core fragment",

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TY - CHAP

T1 - Detection and quantification of hCG and its isoforms with MALDI-ToF mass spectrometry

AU - Zmuidinaite, Raminta

AU - Iles, Ray K.

AU - Pais, Ricardo J.

AU - Butler, Stephen A.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Primarily, the purpose of hCG detection has been the confirmation of pregnancy by analysis of urine or blood. However, the number of applications for which hCG or hCGβ has been used for diagnostic purposes has significantly grown over the years. Examples of such applications are the detection of hydatidiform mole, cancer, ectopic pregnancy, and fetal trisomies. The wide range of applications renders the accurate and precise detection and quantification of the molecule in various biological fluids necessary. Therefore, in this chapter, the quantification and detection of hCG and its variants using MALDI-ToF mass spectrometry will be discussed.

AB - Primarily, the purpose of hCG detection has been the confirmation of pregnancy by analysis of urine or blood. However, the number of applications for which hCG or hCGβ has been used for diagnostic purposes has significantly grown over the years. Examples of such applications are the detection of hydatidiform mole, cancer, ectopic pregnancy, and fetal trisomies. The wide range of applications renders the accurate and precise detection and quantification of the molecule in various biological fluids necessary. Therefore, in this chapter, the quantification and detection of hCG and its variants using MALDI-ToF mass spectrometry will be discussed.

KW - Detection

KW - Immunoassay

KW - MALDI-ToF MS

KW - Quantitation

KW - hCG isoforms

KW - hCGα

KW - hCGβ

KW - hCGβ core fragment

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U2 - 10.1016/B978-0-12-820050-6.00008-4

DO - 10.1016/B978-0-12-820050-6.00008-4

M3 - Chapter

AN - SCOPUS:85117712619

SN - 9780128216095

SP - 75

EP - 85

BT - 100 Years of Human Chorionic Gonadotropin

PB - Elsevier

ER -

Detection and quantification of hCG and its isoforms with MALDI-ToF mass spectrometry

Abstract

Keywords

UN SDGs

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