TY - JOUR
T1 - Determination of glyoxal and methylglyoxal in environmental and biological matrices by stir bar sorptive extraction with in-situ derivatization
AU - Neng, N. R.
AU - Cordeiro, C. A.A.
AU - Freire, A. P.
AU - Nogueira, J. M.F.
PY - 2007/10/26
Y1 - 2007/10/26
N2 - Stir bar sorptive extraction with in-situ derivatization using 2,3-diaminonaphthalene (DAN) followed by liquid desorption and high performance liquid chromatography with diode array detection (SBSE(DAN)in-situ-LD-HPLC-DAD) was developed for the determination of glyoxal (Gly) and methylglyoxal (MGly) in environmental and biological matrices. DAN proved very good specificity as in-situ derivatising agent for Gly and MGly in aqueous media, allowing the formation of adducts with remarkable sensitivity, selectivity and the absence of photodegradation. Assays performed on spiked (1.0 μg L-1) water samples, under convenient experimental conditions, yielded recoveries of 96.2 ± 7.9% for Gly and 96.1 ± 6.4% for MGly. The analytical performance showed good accuracy, suitable precision (<12.0%), low detection limits (15 ng L-1 for Gly and 25 ng L-1 for MGly adducts) and excellent linear dynamic ranges (r2 > 0.99) from 0.1 to 120.0 μg L-1. By using the standard addition method, the application of the present method to tap and swimming-pool water, beer, yeast cells suspension and urine samples allowed very good performance at the trace level. The proposed methodology proved to be a feasible alternative for routine quality control analysis, showing to be easy to implement, reliable, sensitive and with a low sample volume requirement to monitor Gly and MGly in environmental and biological matrices.
AB - Stir bar sorptive extraction with in-situ derivatization using 2,3-diaminonaphthalene (DAN) followed by liquid desorption and high performance liquid chromatography with diode array detection (SBSE(DAN)in-situ-LD-HPLC-DAD) was developed for the determination of glyoxal (Gly) and methylglyoxal (MGly) in environmental and biological matrices. DAN proved very good specificity as in-situ derivatising agent for Gly and MGly in aqueous media, allowing the formation of adducts with remarkable sensitivity, selectivity and the absence of photodegradation. Assays performed on spiked (1.0 μg L-1) water samples, under convenient experimental conditions, yielded recoveries of 96.2 ± 7.9% for Gly and 96.1 ± 6.4% for MGly. The analytical performance showed good accuracy, suitable precision (<12.0%), low detection limits (15 ng L-1 for Gly and 25 ng L-1 for MGly adducts) and excellent linear dynamic ranges (r2 > 0.99) from 0.1 to 120.0 μg L-1. By using the standard addition method, the application of the present method to tap and swimming-pool water, beer, yeast cells suspension and urine samples allowed very good performance at the trace level. The proposed methodology proved to be a feasible alternative for routine quality control analysis, showing to be easy to implement, reliable, sensitive and with a low sample volume requirement to monitor Gly and MGly in environmental and biological matrices.
KW - Beer
KW - Biological fluids
KW - Glyoxal
KW - HPLC-DAD
KW - In-situ derivatization
KW - Methylglyoxal
KW - Stir bar sorptive extraction
KW - Water samples
UR - http://www.scopus.com/inward/record.url?scp=34848875089&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2007.08.060
DO - 10.1016/j.chroma.2007.08.060
M3 - Article
C2 - 17888934
AN - SCOPUS:34848875089
SN - 0021-9673
VL - 1169
SP - 47
EP - 52
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -