TY - JOUR
T1 - Development and application of stir bar sorptive extraction with polyurethane foams for the determination of testosterone and methenolone in urine matrices
AU - Sequeiros, R. C.P.
AU - Neng, N. R.
AU - Portugal, F. C.M.
AU - Pinto, M. L.
AU - Pires, J.
AU - Nogueira, J. M.F.
N1 - Funding Information:
The authors thank Dr. Xavier de la Torre of the National Anti-Doping Laboratory of Portugal for providing the standard solutions of T and Met. Fátima C.M. Portugal (BD/24598/2005) and Nuno R. Neng (BD/38660/2007) wish to thank Fundação para a Ciência e Tecnologia (FCT) for the PhD grants.
PY - 2011/4
Y1 - 2011/4
N2 - This work describes the development, validation, and application of a novel methodology for the determination of testosterone and methenolone in urine matrices by stir bar sorptive extraction using polyurethane foams [SBSE(PU)] followed by liquid desorption and high-performance liquid chromatography with diode array detection. The methodology was optimized in terms of extraction time, agitation speed, pH, ionic strength and organic modifier, as well as back-extraction solvent and desorption time. Under optimized experimental conditions, convenient accuracy were achieved with average recoveries of 49.7 ± 8.6% for testosterone and 54.2 ± 4.7% for methenolone. Additionally, the methodology showed good precision (< 9%), excellent linear dynamic ranges (> 0.9963) and convenient detection limits (0.2-0.3 μg/L). When comparing the efficiency obtained by SBSE(PU) and with the conventional polydimethylsiloxane phase [SBSE(PDMS)], yields up to four-fold higher are attained for the former, under the same experimental conditions. The application of the proposed methodology for the analysis of testosterone and methenolone in urine matrices showed negligible matrix effects and good analytical performance.
AB - This work describes the development, validation, and application of a novel methodology for the determination of testosterone and methenolone in urine matrices by stir bar sorptive extraction using polyurethane foams [SBSE(PU)] followed by liquid desorption and high-performance liquid chromatography with diode array detection. The methodology was optimized in terms of extraction time, agitation speed, pH, ionic strength and organic modifier, as well as back-extraction solvent and desorption time. Under optimized experimental conditions, convenient accuracy were achieved with average recoveries of 49.7 ± 8.6% for testosterone and 54.2 ± 4.7% for methenolone. Additionally, the methodology showed good precision (< 9%), excellent linear dynamic ranges (> 0.9963) and convenient detection limits (0.2-0.3 μg/L). When comparing the efficiency obtained by SBSE(PU) and with the conventional polydimethylsiloxane phase [SBSE(PDMS)], yields up to four-fold higher are attained for the former, under the same experimental conditions. The application of the proposed methodology for the analysis of testosterone and methenolone in urine matrices showed negligible matrix effects and good analytical performance.
UR - http://www.scopus.com/inward/record.url?scp=79953186507&partnerID=8YFLogxK
U2 - 10.1093/chrsci/49.4.297
DO - 10.1093/chrsci/49.4.297
M3 - Article
AN - SCOPUS:79953186507
SN - 0021-9665
VL - 49
SP - 297
EP - 302
JO - Journal of Chromatographic Science
JF - Journal of Chromatographic Science
IS - 4
ER -