TY - JOUR
T1 - Effect of cultivation parameters on the production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and poly(3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate) by Cupriavidus necator using waste glycerol
AU - Cavalheiro, João M.B.T.
AU - Raposo, Rodrigo S.
AU - de Almeida, M. Catarina M.D.
AU - Teresa Cesário, M.
AU - Sevrin, Chantal
AU - Grandfils, Christian
AU - da Fonseca, M. M.R.
N1 - Funding Information:
This work was partially financed by the EU Integrated Project BIOPRODUCTION (Contract No. 026515-2). João Cavalheiro, Catarina Almeida and Teresa Cesário are supported by fellowships (SFRH/BD/45266/2008), (SFRH/BPD/26678/2006) and (SFRH/BPD/68587/2010), respectively, from Fundação para a Ciência e Tecnologia, Portugal. We are grateful to Dr. P. Leitão and to Fábrica Torrejana de Biocombustíveis, S.A., Torres Vedras, Portugal, for the GRP supply.
PY - 2012/5
Y1 - 2012/5
N2 - Short-chain polyhydroxyalkanoate co-polymers (poly(3-hydroxybutyrate-co-4-hydroxybutyrate)) (P(3HB-co-4HB)) and terpolymers (poly(3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate)) (P(3HB-4HB-3HV)) were produced using high-cell density fed-batch cultures of Cupriavidus necator DSM 545. C-source for growth and 3HB synthesis was waste glycerol (GRP) from a biodiesel plant. Incorporation of 4HB monomers was promoted by γ-butyrolactone (GBL). Propionic acid (PA), a stimulator of 4HB accumulation, increased the 4HB molar ratio 2-fold, but also acted as 3HV precursor, yielding P(3HB-4HB-3HV). Dissolved oxygen (DOC) was a key parameter for % PHA accumulation and volumetric productivity (Prod vol). 4HB molar ratio increased in the presence of PA and with extended accumulation time. By manipulating DOC and cultivation time, P(3HB-4HB) with between 11.4 and 21.5molar% of 4HB were attained. Similarly, P(3HB-4HB-3HV) was obtained with 4HB molar% between 24.8% and 43.6% and 3HV% from 5.6% to 9.8%. Mw varied between 5.5×10 5 and 1.37×10 6Da. PHA production from GRP helps reducing production costs with concomitant GRP valorization.
AB - Short-chain polyhydroxyalkanoate co-polymers (poly(3-hydroxybutyrate-co-4-hydroxybutyrate)) (P(3HB-co-4HB)) and terpolymers (poly(3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate)) (P(3HB-4HB-3HV)) were produced using high-cell density fed-batch cultures of Cupriavidus necator DSM 545. C-source for growth and 3HB synthesis was waste glycerol (GRP) from a biodiesel plant. Incorporation of 4HB monomers was promoted by γ-butyrolactone (GBL). Propionic acid (PA), a stimulator of 4HB accumulation, increased the 4HB molar ratio 2-fold, but also acted as 3HV precursor, yielding P(3HB-4HB-3HV). Dissolved oxygen (DOC) was a key parameter for % PHA accumulation and volumetric productivity (Prod vol). 4HB molar ratio increased in the presence of PA and with extended accumulation time. By manipulating DOC and cultivation time, P(3HB-4HB) with between 11.4 and 21.5molar% of 4HB were attained. Similarly, P(3HB-4HB-3HV) was obtained with 4HB molar% between 24.8% and 43.6% and 3HV% from 5.6% to 9.8%. Mw varied between 5.5×10 5 and 1.37×10 6Da. PHA production from GRP helps reducing production costs with concomitant GRP valorization.
KW - Poly(3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate)
KW - Poly(3-hydroxybutyrate-co-4-hydroxybutyrate)
KW - Waste glycerol
UR - http://www.scopus.com/inward/record.url?scp=84858747436&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2012.01.176
DO - 10.1016/j.biortech.2012.01.176
M3 - Article
C2 - 22382294
AN - SCOPUS:84858747436
SN - 0960-8524
VL - 111
SP - 391
EP - 397
JO - Bioresource Technology
JF - Bioresource Technology
ER -