TY - JOUR
T1 - Electrochemical studies of rubredoxin from desulfovibrio vulgaris at modified electrodes
AU - Correia dos Santos, M. M.
AU - Paes de Sousa, P. M.
AU - Simõoes Goncçalves, M. L.
AU - Ascenso, C.
AU - Moura, I.
AU - Moura, J. J.G.
N1 - Funding Information:
This work is within the research project Praxis 2/2.1/QUI/312/94. P. Sousa thanks FCT for financial support.
PY - 2001/3/23
Y1 - 2001/3/23
N2 - The electrochemical behaviour of rubredoxin from Desulfovibrio vulgaris is analysed in 0.1M KCl at pH7.6±0.1 (10 mM Tris buffer) using gold disk electrodes (r=12.5 μm and r=0.08 cm) in the presence of Mg2+ and/or neomycin, and revisited using a carbon electrode (r=0.15cm) in the presence of Mg2+ and further analysed in solutions with neomycin with and without Mg2+. The results show that, depending on the modifier and its concentration, the electrode surface may have different densities of electroactive sites and so different mass transport processes may take place, thus accounting for the shape of the voltammograms. Values for ks, D and E°′ determined under all conditions agreed with each other within experimental error, no matter what the size or material of the electrode or the modifier used.
AB - The electrochemical behaviour of rubredoxin from Desulfovibrio vulgaris is analysed in 0.1M KCl at pH7.6±0.1 (10 mM Tris buffer) using gold disk electrodes (r=12.5 μm and r=0.08 cm) in the presence of Mg2+ and/or neomycin, and revisited using a carbon electrode (r=0.15cm) in the presence of Mg2+ and further analysed in solutions with neomycin with and without Mg2+. The results show that, depending on the modifier and its concentration, the electrode surface may have different densities of electroactive sites and so different mass transport processes may take place, thus accounting for the shape of the voltammograms. Values for ks, D and E°′ determined under all conditions agreed with each other within experimental error, no matter what the size or material of the electrode or the modifier used.
KW - Modified macro- and micro-electrodes
KW - Rubredoxin
KW - Unmediated electrochemistry
UR - http://www.scopus.com/inward/record.url?scp=0035937233&partnerID=8YFLogxK
U2 - 10.1016/S0022-0728(00)00521-0
DO - 10.1016/S0022-0728(00)00521-0
M3 - Article
AN - SCOPUS:0035937233
SN - 0022-0728
VL - 501
SP - 173
EP - 179
JO - Journal of Electroanalytical Chemistry
JF - Journal of Electroanalytical Chemistry
IS - 1-2
ER -