Electron transfer and docking between cytochrome cd1 nitrite reductase and different redox partners - A comparative study

Humberto A. Pedroso, Célia M. Silveira, Rui M. Almeida, Ana Almeida, Stéphane Besson, Isabel Moura, José J.G. Moura, M. Gabriela Almeida

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Cytochrome cd1 nitrite reductases (cd1NiRs) catalyze the reduction of nitrite to nitric oxide in denitrifying bacteria, such as Marinobacter hydrocarbonoclasticus. Previous work demonstrated that the enzymatic activity depends on a structural pre-activation triggered by the entry of electrons through the electron transfer (ET) domain, which houses a heme c center. The catalytic activity of M. hydrocarbonoclasticus cd1NiR (Mhcd1NiR) was tested by mediated electrochemistry, using small ET proteins and chemical redox mediators. The rate of enzymatic reaction depends on the nature of the redox partner, with cytochrome (cyt) c552 providing the highest value. In situations where cyt c552 is replaced by either a biological (cyt c from horse heart) or a chemical mediator the catalytic response was only observed at very low scan rates, suggesting that the intermolecular ET rate is much slower. Molecular docking simulations with the 3D model structure of Mhcd1NiR and cyt c552 or cyt c showed that hydrophobic interactions favor the formation of complexes where the heme c domain of the enzyme is the principal docking site. However, only in the case of cyt c552 the preferential areas of contact and Fe-Fe distances between heme c groups of the redox partners allow establishing competent ET pathways. The coupling of the enzyme with chemical redox mediators was also found not to be energetically favorable. These results indicate that although low activity functional complexes can be formed between Mhcd1NiR and different types of redox mediators, efficient ET is only observed with the putative physiological electron donor cyt c552.

Original languageEnglish
Pages (from-to)1412-1421
Number of pages10
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1857
Issue number9
DOIs
Publication statusPublished - 1 Sept 2016

Keywords

  • Cytochrome c
  • Cytochrome cd nitrite reductase
  • Electronic pathways
  • Intermolecular electron transfer
  • Mediated electrochemistry
  • Molecular coupling

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