Enhanced Direct Electron Transfer of a Multihemic Nitrite Reductase on Single-walled Carbon Nanotube Modified Electrodes

Célia M. Silveira; Jessica Baur; Michael Holzinger; José J.G. Moura; Serge Cosnier; M. Gabriela Almeida

doi:10.1002/elan.201000363

Enhanced Direct Electron Transfer of a Multihemic Nitrite Reductase on Single-walled Carbon Nanotube Modified Electrodes

Célia M. Silveira, Jessica Baur, Michael Holzinger, José J.G. Moura, Serge Cosnier, M. Gabriela Almeida

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Single-walled carbon nanotubes (SWCNTs) deposits on glassy carbon and pyrolytic graphite electrodes have dramatically enhanced the direct electron transfer of the multihemic nitrite reductase from Desulfovibrio desulfuricans ATCC 27774, enabling a 10-fold increase in catalytic currents. At optimal conditions, the sensitivity to nitrite and the maximum current density were 2.4±0.1A Lmol^-1cm^-2 and 1500μA cm^-2, respectively. Since the biosensor performance decreased over time, laponite clay and electropolymerized amphiphilic pyrrole were tested as protecting layers. Both coating materials increased substantially the bioelectrode stability, which kept about 90% and 60 % of its initial sensitivity to nitrite after 20 and 248 days, respectively.

Original language	English
Pages (from-to)	2973-2978
Number of pages	6
Journal	Electroanalysis
Volume	22
Issue number	24
DOIs	https://doi.org/10.1002/elan.201000363
Publication status	Published - Dec 2010

Keywords

Carbon nanotubes
Clay
Direct electron transfer
Nitrite reductase
Polypyrrole

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10.1002/elan.201000363

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abstract = "Single-walled carbon nanotubes (SWCNTs) deposits on glassy carbon and pyrolytic graphite electrodes have dramatically enhanced the direct electron transfer of the multihemic nitrite reductase from Desulfovibrio desulfuricans ATCC 27774, enabling a 10-fold increase in catalytic currents. At optimal conditions, the sensitivity to nitrite and the maximum current density were 2.4±0.1A Lmol-1cm-2 and 1500μA cm-2, respectively. Since the biosensor performance decreased over time, laponite clay and electropolymerized amphiphilic pyrrole were tested as protecting layers. Both coating materials increased substantially the bioelectrode stability, which kept about 90% and 60 % of its initial sensitivity to nitrite after 20 and 248 days, respectively.",

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AU - Silveira, Célia M.

AU - Baur, Jessica

AU - Holzinger, Michael

AU - Moura, José J.G.

AU - Cosnier, Serge

AU - Almeida, M. Gabriela

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AB - Single-walled carbon nanotubes (SWCNTs) deposits on glassy carbon and pyrolytic graphite electrodes have dramatically enhanced the direct electron transfer of the multihemic nitrite reductase from Desulfovibrio desulfuricans ATCC 27774, enabling a 10-fold increase in catalytic currents. At optimal conditions, the sensitivity to nitrite and the maximum current density were 2.4±0.1A Lmol-1cm-2 and 1500μA cm-2, respectively. Since the biosensor performance decreased over time, laponite clay and electropolymerized amphiphilic pyrrole were tested as protecting layers. Both coating materials increased substantially the bioelectrode stability, which kept about 90% and 60 % of its initial sensitivity to nitrite after 20 and 248 days, respectively.

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Enhanced Direct Electron Transfer of a Multihemic Nitrite Reductase on Single-walled Carbon Nanotube Modified Electrodes

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Keywords

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