TY - JOUR
T1 - Enzyme purification with aqueous two-phase systems
T2 - Comparison between systems composed of pure polymers and systems composed of crude polymers
AU - Venâncio, Armando
AU - Almeida, Catarina
AU - Teixeira, José A.
N1 - Funding Information:
was supported by a JNICT (Junta Investigaqfio Cientffica e Tecno-
PY - 1996/5/17
Y1 - 1996/5/17
N2 - The main drawback when using aqueous two-phase systems for macromolecule purification is the high cost of most polymers used. The purification of an enzyme, alcohol dehydrogenase, from a crude extract of Saccharomyces cerevisiae was tested in systems composed of poly(ethylene glycol) and a crude hydroxypropyl starch or Reppal PES 100, a purified fraction of hydroxypropyl starch. Purification factors measured for the enzyme were very similar in both systems (between 0.8 and 1.4 for both systems in the upper phase). However, systems composed of Reppal PES present a greater recovery of enzyme, between 77% and 100% versus 60% and 100%, while systems composed of crude hydroxypropyl starch exhibit a larger Δlog K for the rested ligand, 1.26 versus 0.81.
AB - The main drawback when using aqueous two-phase systems for macromolecule purification is the high cost of most polymers used. The purification of an enzyme, alcohol dehydrogenase, from a crude extract of Saccharomyces cerevisiae was tested in systems composed of poly(ethylene glycol) and a crude hydroxypropyl starch or Reppal PES 100, a purified fraction of hydroxypropyl starch. Purification factors measured for the enzyme were very similar in both systems (between 0.8 and 1.4 for both systems in the upper phase). However, systems composed of Reppal PES present a greater recovery of enzyme, between 77% and 100% versus 60% and 100%, while systems composed of crude hydroxypropyl starch exhibit a larger Δlog K for the rested ligand, 1.26 versus 0.81.
KW - Alcohol dehydrogenase
KW - Enzyme
KW - Polymer
UR - http://www.scopus.com/inward/record.url?scp=0342592294&partnerID=8YFLogxK
U2 - 10.1016/0378-4347(95)00419-X
DO - 10.1016/0378-4347(95)00419-X
M3 - Article
C2 - 8798890
AN - SCOPUS:0342592294
SN - 1572-6495
VL - 680
SP - 131
EP - 136
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1-2
ER -