Expansion and neural differentiation of embryonic stem cells in adherent and suspension cultures

Elsa Albranches; Evguenia Bekman; Domingos Henrique; Joaquim M.S. Cabral

doi:10.1023/A:1023462832608

Expansion and neural differentiation of embryonic stem cells in adherent and suspension cultures

Elsa Albranches, Evguenia Bekman, Domingos Henrique, Joaquim M.S. Cabral

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The embryonic stem cell line, S25, is a genetically modified line that allows lineage selection of neural cells (M. Li, L. Lovell-Badge, A. Smith (1998) Current Biology 8: 971-974). Here, the growth parameters of this cell line were analysed. Serial passaging in adherent conditions enabled these cells to grow rapidly (average specific growth rates of 0.035 h^-1) and generate high viable cell densities (above 90%). The aggregation of the S25 cells into embryoid bodies (EBs) was also studied, indicating limited cell growth (maximum cell densities of 2.7 × 10⁵ cells ml^-1) and a high variability of aggregate size (70-400 μm after 8 d). Enzymatic dissociation of EBs with 1% (v/v) trypsin gave highest cell viability (91%) and density (1.4 × 10⁴ cells m1^-1) and the cells thus obtained are able to differentiate into neurons.

Original language	English
Pages (from-to)	725-730
Number of pages	6
Journal	Biotechnology Letters
Volume	25
Issue number	9
DOIs	https://doi.org/10.1023/A:1023462832608
Publication status	Published - May 2003
Externally published	Yes

Keywords

Embryoid bodies
Embryonic stem cells
Expansion
Growth conditions

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title = "Expansion and neural differentiation of embryonic stem cells in adherent and suspension cultures",

abstract = "The embryonic stem cell line, S25, is a genetically modified line that allows lineage selection of neural cells (M. Li, L. Lovell-Badge, A. Smith (1998) Current Biology 8: 971-974). Here, the growth parameters of this cell line were analysed. Serial passaging in adherent conditions enabled these cells to grow rapidly (average specific growth rates of 0.035 h-1) and generate high viable cell densities (above 90%). The aggregation of the S25 cells into embryoid bodies (EBs) was also studied, indicating limited cell growth (maximum cell densities of 2.7 × 105 cells ml-1) and a high variability of aggregate size (70-400 μm after 8 d). Enzymatic dissociation of EBs with 1% (v/v) trypsin gave highest cell viability (91%) and density (1.4 × 104 cells m1-1) and the cells thus obtained are able to differentiate into neurons.",

keywords = "Embryoid bodies, Embryonic stem cells, Expansion, Growth conditions",

author = "Elsa Albranches and Evguenia Bekman and Domingos Henrique and Cabral, {Joaquim M.S.}",

note = "Funding Information: We thank Meng Li and Austin Smith for the gift of S25 cells and for help along the work. E.A. and E.B. are supported by fellowships from Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia, Portugal (BD/6185/2001 and BPD/18857/98). This work was supported by Fun-da{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia, Portugal (grants BIA/12213/98 and POCTI/NSE/34622/99).",

year = "2003",

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doi = "10.1023/A:1023462832608",

language = "English",

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AU - Albranches, Elsa

AU - Bekman, Evguenia

AU - Henrique, Domingos

AU - Cabral, Joaquim M.S.

N1 - Funding Information: We thank Meng Li and Austin Smith for the gift of S25 cells and for help along the work. E.A. and E.B. are supported by fellowships from Fundação para a Ciência e a Tecnologia, Portugal (BD/6185/2001 and BPD/18857/98). This work was supported by Fun-dação para a Ciência e a Tecnologia, Portugal (grants BIA/12213/98 and POCTI/NSE/34622/99).

PY - 2003/5

Y1 - 2003/5

N2 - The embryonic stem cell line, S25, is a genetically modified line that allows lineage selection of neural cells (M. Li, L. Lovell-Badge, A. Smith (1998) Current Biology 8: 971-974). Here, the growth parameters of this cell line were analysed. Serial passaging in adherent conditions enabled these cells to grow rapidly (average specific growth rates of 0.035 h-1) and generate high viable cell densities (above 90%). The aggregation of the S25 cells into embryoid bodies (EBs) was also studied, indicating limited cell growth (maximum cell densities of 2.7 × 105 cells ml-1) and a high variability of aggregate size (70-400 μm after 8 d). Enzymatic dissociation of EBs with 1% (v/v) trypsin gave highest cell viability (91%) and density (1.4 × 104 cells m1-1) and the cells thus obtained are able to differentiate into neurons.

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Expansion and neural differentiation of embryonic stem cells in adherent and suspension cultures

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Keywords

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