TY - JOUR
T1 - Expansion and neural differentiation of embryonic stem cells in adherent and suspension cultures
AU - Albranches, Elsa
AU - Bekman, Evguenia
AU - Henrique, Domingos
AU - Cabral, Joaquim M.S.
N1 - Funding Information:
We thank Meng Li and Austin Smith for the gift of S25 cells and for help along the work. E.A. and E.B. are supported by fellowships from Fundação para a Ciência e a Tecnologia, Portugal (BD/6185/2001 and BPD/18857/98). This work was supported by Fun-dação para a Ciência e a Tecnologia, Portugal (grants BIA/12213/98 and POCTI/NSE/34622/99).
PY - 2003/5
Y1 - 2003/5
N2 - The embryonic stem cell line, S25, is a genetically modified line that allows lineage selection of neural cells (M. Li, L. Lovell-Badge, A. Smith (1998) Current Biology 8: 971-974). Here, the growth parameters of this cell line were analysed. Serial passaging in adherent conditions enabled these cells to grow rapidly (average specific growth rates of 0.035 h-1) and generate high viable cell densities (above 90%). The aggregation of the S25 cells into embryoid bodies (EBs) was also studied, indicating limited cell growth (maximum cell densities of 2.7 × 105 cells ml-1) and a high variability of aggregate size (70-400 μm after 8 d). Enzymatic dissociation of EBs with 1% (v/v) trypsin gave highest cell viability (91%) and density (1.4 × 104 cells m1-1) and the cells thus obtained are able to differentiate into neurons.
AB - The embryonic stem cell line, S25, is a genetically modified line that allows lineage selection of neural cells (M. Li, L. Lovell-Badge, A. Smith (1998) Current Biology 8: 971-974). Here, the growth parameters of this cell line were analysed. Serial passaging in adherent conditions enabled these cells to grow rapidly (average specific growth rates of 0.035 h-1) and generate high viable cell densities (above 90%). The aggregation of the S25 cells into embryoid bodies (EBs) was also studied, indicating limited cell growth (maximum cell densities of 2.7 × 105 cells ml-1) and a high variability of aggregate size (70-400 μm after 8 d). Enzymatic dissociation of EBs with 1% (v/v) trypsin gave highest cell viability (91%) and density (1.4 × 104 cells m1-1) and the cells thus obtained are able to differentiate into neurons.
KW - Embryoid bodies
KW - Embryonic stem cells
KW - Expansion
KW - Growth conditions
UR - http://www.scopus.com/inward/record.url?scp=0037680702&partnerID=8YFLogxK
U2 - 10.1023/A:1023462832608
DO - 10.1023/A:1023462832608
M3 - Article
C2 - 12882174
AN - SCOPUS:0037680702
SN - 0141-5492
VL - 25
SP - 725
EP - 730
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 9
ER -