Abstract
Extraction of a steroid 1,2 dehydrogenase was achieved by sonication after stabilization by the addition of monohydrated sodium glutamate (1.5 M). A specific first order rate of enzyme release of 0.035 min-1 was obtained. Studies were carried out on the following sonication parameters: power of sonication, cell concentration and container volume. A total recirculating system appears to be an advantageous approach to process larger volumes.
| Original language | English |
|---|---|
| Pages (from-to) | 271-278 |
| Number of pages | 8 |
| Journal | Bioseparation |
| Volume | 4 |
| Issue number | 4 |
| Publication status | Published - Aug 1994 |
| Externally published | Yes |