Extraction of a steroid 1,2 dehydrogenase by sonication of Arthrobacter simplex cells

J. C.G. Caldeira; J. M.S. Cabral

Extraction of a steroid 1,2 dehydrogenase by sonication of Arthrobacter simplex cells

J. C.G. Caldeira
, J. M.S. Cabral

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Extraction of a steroid 1,2 dehydrogenase was achieved by sonication after stabilization by the addition of monohydrated sodium glutamate (1.5 M). A specific first order rate of enzyme release of 0.035 min^-1 was obtained. Studies were carried out on the following sonication parameters: power of sonication, cell concentration and container volume. A total recirculating system appears to be an advantageous approach to process larger volumes.

Original language	English
Pages (from-to)	271-278
Number of pages	8
Journal	Bioseparation
Volume	4
Issue number	4
Publication status	Published - Aug 1994
Externally published	Yes

Cite this

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title = "Extraction of a steroid 1,2 dehydrogenase by sonication of Arthrobacter simplex cells",

abstract = "Extraction of a steroid 1,2 dehydrogenase was achieved by sonication after stabilization by the addition of monohydrated sodium glutamate (1.5 M). A specific first order rate of enzyme release of 0.035 min-1 was obtained. Studies were carried out on the following sonication parameters: power of sonication, cell concentration and container volume. A total recirculating system appears to be an advantageous approach to process larger volumes.",

author = "Caldeira, \{J. C.G.\} and Cabral, \{J. M.S.\}",

year = "1994",

month = aug,

language = "English",

volume = "4",

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journal = "Bioseparation",

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publisher = "Kluwer Academic Publishers",

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T1 - Extraction of a steroid 1,2 dehydrogenase by sonication of Arthrobacter simplex cells

AU - Caldeira, J. C.G.

AU - Cabral, J. M.S.

PY - 1994/8

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N2 - Extraction of a steroid 1,2 dehydrogenase was achieved by sonication after stabilization by the addition of monohydrated sodium glutamate (1.5 M). A specific first order rate of enzyme release of 0.035 min-1 was obtained. Studies were carried out on the following sonication parameters: power of sonication, cell concentration and container volume. A total recirculating system appears to be an advantageous approach to process larger volumes.

AB - Extraction of a steroid 1,2 dehydrogenase was achieved by sonication after stabilization by the addition of monohydrated sodium glutamate (1.5 M). A specific first order rate of enzyme release of 0.035 min-1 was obtained. Studies were carried out on the following sonication parameters: power of sonication, cell concentration and container volume. A total recirculating system appears to be an advantageous approach to process larger volumes.

UR - https://www.scopus.com/pages/publications/0028485766

M3 - Article

AN - SCOPUS:0028485766

SN - 0923-179X

VL - 4

SP - 271

EP - 278

JO - Bioseparation

JF - Bioseparation

IS - 4

ER -

Extraction of a steroid 1,2 dehydrogenase by sonication of Arthrobacter simplex cells

Abstract

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