TY - JOUR
T1 - Fast LC-MS/MS method for the determination of amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in urine
AU - Concheiro, Marta
AU - Simões, Susana Maria dos Santos Sadler
AU - Quintela, Óscar
AU - de Castro, Ana
AU - Dias, Mário João Rodrigues
AU - Cruz, Angelines
AU - López-Rivadulla, Manuel
N1 - Funding Information:
This work was done thanks to the financial support from the Ministerio de Educación y Ciencia of Spain (F.P.U. Grant numbers AP-2002-2935 and AP-2002-2878).
PY - 2007/8/24
Y1 - 2007/8/24
N2 - A fast method was designed for the simultaneous determination of amphetamine (A), methamphetamine (MA), PMA, MDA, MDMA, MDEA and MBDB in urine. The drugs were analysed by LC (ESI)-MS/MS, after a simple liquid-liquid extraction in the presence of the deuterated analogues. Reverse phase separation on an Atlantis dC18 Intelligent Speed column was achieved in less than 4 min under gradient conditions, and the total run time was 8 min. The method was fully validated, including linearity (1-1000 ng/mL for A, MDMA, MDEA and MBDB; 2-1000 ng/mL for MDA and PMA; 1-200 ng/mL for MA; r2 > 0.99 for all compounds), recovery (>80%), within-day and between-day precision and accuracy (CV and MRE < 12.7% for intermediate level and ULOQ, and <17.2% for LLOQ), limit of detection (0.2 ng/mL for MDMA, MDEA and MBDB; 0.5 ng/mL for A, MA and PMA; 1 ng/mL for MDA) and quantitation (1 ng/mL for A, MA, MDMA, MDEA and MBDB; 2 ng/mL for MDA and PMA) and relative ion intensities. No matrix effect was observed. The procedure proved to be sensitive, specific and rapid, and was applied to real forensic cases.
AB - A fast method was designed for the simultaneous determination of amphetamine (A), methamphetamine (MA), PMA, MDA, MDMA, MDEA and MBDB in urine. The drugs were analysed by LC (ESI)-MS/MS, after a simple liquid-liquid extraction in the presence of the deuterated analogues. Reverse phase separation on an Atlantis dC18 Intelligent Speed column was achieved in less than 4 min under gradient conditions, and the total run time was 8 min. The method was fully validated, including linearity (1-1000 ng/mL for A, MDMA, MDEA and MBDB; 2-1000 ng/mL for MDA and PMA; 1-200 ng/mL for MA; r2 > 0.99 for all compounds), recovery (>80%), within-day and between-day precision and accuracy (CV and MRE < 12.7% for intermediate level and ULOQ, and <17.2% for LLOQ), limit of detection (0.2 ng/mL for MDMA, MDEA and MBDB; 0.5 ng/mL for A, MA and PMA; 1 ng/mL for MDA) and quantitation (1 ng/mL for A, MA, MDMA, MDEA and MBDB; 2 ng/mL for MDA and PMA) and relative ion intensities. No matrix effect was observed. The procedure proved to be sensitive, specific and rapid, and was applied to real forensic cases.
KW - Amphetamine
KW - LC-MS/MS
KW - MDMA
KW - PMA
KW - Urine
UR - http://www.scopus.com/inward/record.url?scp=34547597375&partnerID=8YFLogxK
U2 - 10.1016/j.forsciint.2006.10.004
DO - 10.1016/j.forsciint.2006.10.004
M3 - Article
C2 - 17097252
AN - SCOPUS:34547597375
SN - 0379-0738
VL - 171
SP - 44
EP - 51
JO - Forensic Science International
JF - Forensic Science International
IS - 1
ER -