Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by [ZnCr-AQS] LDH

H. Chen; C. Mousty; S. Cosnier; C. Silveira; J. J.G. Moura; M. G. Almeida

doi:10.1016/j.elecom.2007.05.030

Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by [ZnCr-AQS] LDH

H. Chen, C. Mousty, S. Cosnier, C. Silveira, J. J.G. Moura, M. G. Almeida

Research output: Contribution to journal › Article › peer-review

83 Citations (Scopus)

Abstract

A biosensor for amperometric determination of nitrite was developed using cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans immobilized and electrically connected on a glassy carbon electrode by entrapment into redox active [ZnCr-AQS] layered double hydroxide containing anthraquinone-2-sulfonate (AQS). The transduction step corresponded to the electro-enzymatic reduction of nitrite by immobilized AQS molecules at -0.6 V. The biosensor showed a fast response to nitrite (5 s) with a linear range between 0.015 and 2.35 μM, a sensitivity of 1.8 A M^-1 cm^-2 and a detection limit of 4 nM. The apparent Michaelis-Menten constant (K_M^app) was 7.5 μM.

Original language	English
Pages (from-to)	2240-2245
Number of pages	6
Journal	Electrochemistry Communications
Volume	9
Issue number	9
DOIs	https://doi.org/10.1016/j.elecom.2007.05.030
Publication status	Published - Sept 2007

Keywords

Anthraquinone sulfonate
Biosensor
Layered double hydroxides
Nitrite
Nitrite reductase

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10.1016/j.elecom.2007.05.030

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@article{dc83628a96fa4141a5e6d70a5ecf51c8,

title = "Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by [ZnCr-AQS] LDH",

abstract = "A biosensor for amperometric determination of nitrite was developed using cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans immobilized and electrically connected on a glassy carbon electrode by entrapment into redox active [ZnCr-AQS] layered double hydroxide containing anthraquinone-2-sulfonate (AQS). The transduction step corresponded to the electro-enzymatic reduction of nitrite by immobilized AQS molecules at -0.6 V. The biosensor showed a fast response to nitrite (5 s) with a linear range between 0.015 and 2.35 μM, a sensitivity of 1.8 A M-1 cm-2 and a detection limit of 4 nM. The apparent Michaelis-Menten constant (KMapp) was 7.5 μM.",

keywords = "Anthraquinone sulfonate, Biosensor, Layered double hydroxides, Nitrite, Nitrite reductase",

author = "H. Chen and C. Mousty and S. Cosnier and C. Silveira and Moura, \{J. J.G.\} and Almeida, \{M. G.\}",

note = "Funding Information: This work was done in the frame work of the Programme CNRS-GRICES France-Portugal 2005 and the Programme de Recherche en R{\'e}seaux Franco-Chinois P2R “Eau et D{\'e}veloppement durable” (2004–2007). Hao Chen acknowledges the French Ministry of Research and New Technologies for supporting her postdoctoral stay in Grenoble.",

year = "2007",

month = sep,

doi = "10.1016/j.elecom.2007.05.030",

language = "English",

volume = "9",

pages = "2240--2245",

journal = "Electrochemistry Communications",

issn = "1388-2481",

publisher = "Elsevier Inc.",

number = "9",

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TY - JOUR

T1 - Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by [ZnCr-AQS] LDH

AU - Chen, H.

AU - Mousty, C.

AU - Cosnier, S.

AU - Silveira, C.

AU - Moura, J. J.G.

AU - Almeida, M. G.

N1 - Funding Information: This work was done in the frame work of the Programme CNRS-GRICES France-Portugal 2005 and the Programme de Recherche en Réseaux Franco-Chinois P2R “Eau et Développement durable” (2004–2007). Hao Chen acknowledges the French Ministry of Research and New Technologies for supporting her postdoctoral stay in Grenoble.

PY - 2007/9

Y1 - 2007/9

N2 - A biosensor for amperometric determination of nitrite was developed using cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans immobilized and electrically connected on a glassy carbon electrode by entrapment into redox active [ZnCr-AQS] layered double hydroxide containing anthraquinone-2-sulfonate (AQS). The transduction step corresponded to the electro-enzymatic reduction of nitrite by immobilized AQS molecules at -0.6 V. The biosensor showed a fast response to nitrite (5 s) with a linear range between 0.015 and 2.35 μM, a sensitivity of 1.8 A M-1 cm-2 and a detection limit of 4 nM. The apparent Michaelis-Menten constant (KMapp) was 7.5 μM.

AB - A biosensor for amperometric determination of nitrite was developed using cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans immobilized and electrically connected on a glassy carbon electrode by entrapment into redox active [ZnCr-AQS] layered double hydroxide containing anthraquinone-2-sulfonate (AQS). The transduction step corresponded to the electro-enzymatic reduction of nitrite by immobilized AQS molecules at -0.6 V. The biosensor showed a fast response to nitrite (5 s) with a linear range between 0.015 and 2.35 μM, a sensitivity of 1.8 A M-1 cm-2 and a detection limit of 4 nM. The apparent Michaelis-Menten constant (KMapp) was 7.5 μM.

KW - Anthraquinone sulfonate

KW - Biosensor

KW - Layered double hydroxides

KW - Nitrite

KW - Nitrite reductase

UR - https://www.scopus.com/pages/publications/34548019988

U2 - 10.1016/j.elecom.2007.05.030

DO - 10.1016/j.elecom.2007.05.030

M3 - Article

AN - SCOPUS:34548019988

SN - 1388-2481

VL - 9

SP - 2240

EP - 2245

JO - Electrochemistry Communications

JF - Electrochemistry Communications

IS - 9

ER -

Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by [ZnCr-AQS] LDH

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Keywords

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