TY - JOUR
T1 - Immunodominant Molecules for the Immunodiagnosis of Strongyloides stercoralis Infection
AU - Oliveira, Cíntia de Lima
AU - de Souza, Joelma Nascimento
AU - Barreto, Nilo Manoel Pereira Vieira
AU - Araújo, Weslei Almeida Costa
AU - Sousa, Joana R.
AU - Maraux, Victor Amazonas Pereira
AU - Pinheiro, Carina da Silva
AU - Almeida, Maria Gabriela
AU - Teixeira, Márcia Cristina Aquino
AU - Soares, Neci Matos
N1 - Publisher Copyright:
© 2024
PY - 2025/3
Y1 - 2025/3
N2 - This study aimed to identify immunodominant molecules with potential for S. stercoralis infection immunodiagnosis. Serum samples were tested by an IgG-ELISA using Strongyloides venezuelensis soluble (AgS) and membrane (AgM) antigens, and its protein fractions (<100 and >100 kDa). Proteins were tested by one- and two-dimensional (2D) gel immunoblotting. The ELISA results showed sensitivities, ranging from 82.3 to 85.3 %, while specificities ranged from 60.0 to 93.3 %. Cross-reactivities were observed with sera from individuals infected with other helminths. Immunoreactive protein bands were revealed by Western blotting using both antigens, and a band with molecular weight around 140 kDa of AgS showed 100 % sensitivity and specificity, without cross-reactivity. The 2D immunoblotting revealed 7 spots in the 140 kDa protein with the S. stercoralis pool of serums. The pre-selection of Strongyloides immunodominant proteins that are highly specific and free from cross-reactions is essential for the production of recombinant antigens for the immunodiagnosis of strongyloidiasis.
AB - This study aimed to identify immunodominant molecules with potential for S. stercoralis infection immunodiagnosis. Serum samples were tested by an IgG-ELISA using Strongyloides venezuelensis soluble (AgS) and membrane (AgM) antigens, and its protein fractions (<100 and >100 kDa). Proteins were tested by one- and two-dimensional (2D) gel immunoblotting. The ELISA results showed sensitivities, ranging from 82.3 to 85.3 %, while specificities ranged from 60.0 to 93.3 %. Cross-reactivities were observed with sera from individuals infected with other helminths. Immunoreactive protein bands were revealed by Western blotting using both antigens, and a band with molecular weight around 140 kDa of AgS showed 100 % sensitivity and specificity, without cross-reactivity. The 2D immunoblotting revealed 7 spots in the 140 kDa protein with the S. stercoralis pool of serums. The pre-selection of Strongyloides immunodominant proteins that are highly specific and free from cross-reactions is essential for the production of recombinant antigens for the immunodiagnosis of strongyloidiasis.
KW - ELISA
KW - Immunoblotting
KW - Immunodiagnosis
KW - Immunodominant antigens
KW - Strongyloides stercoralis
UR - http://www.scopus.com/inward/record.url?scp=85212860053&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2024.116649
DO - 10.1016/j.diagmicrobio.2024.116649
M3 - Article
AN - SCOPUS:85212860053
SN - 0732-8893
VL - 111
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 3
M1 - 116649
ER -