Immunolocalization of Notch Signaling in Mouse Preimplantation Embryos

Elisabete Silva, Patrícia Diniz, Alexandre Trindade, Mariana Batista, Ana Torres, António Duarte, Luís Lopes-da-Costa

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

The Notch signaling pathway is an important determinant of cell diversity and identity in most developing embryonic tissues. The pathway components are expressed dynamically, and their function is critical for embryonic survival. This protocol addresses the immunolocalization of Notch pathway components by confocal microscopy.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages173-185
Number of pages13
DOIs
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2472
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Confocal microscopy
  • Immunocytochemistry
  • Mouse
  • Notch intracellular domain (NICD)
  • Notch signaling
  • Preimplantation embryo

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