Abstract
The polymerase chain reaction with degenerate primers corresponding to the most conservative amino acids 16-21 (ELEKEF) and 49-54 (WFQNRR) of the Antennapedia class homeodomain was used for the amplification of cDNA from regenerating planarians (asexual race of Dugesia tigrina). A total of six new Antennapedia-like homeobox sequences, designated Dutarh-1-Dutarh-6 (Dugesia tigrina asexual race homeobox gene), were obtained. Their comparison with other homeobox genes using a "Genebee" software (the EMBL Data Library) showed that all sequences except Dutarh-6 belong to the Antennapedia class. Dutarh-6 is closely related to a recently described novel homeobox gene subfamily that includes mouse mesodermal homeobox genes Mox-1 and Mox-2 and rat homeobox gene Gax.
| Translated title of the contribution | The cloning of fragments of homeobox genes expressed during planarian regeneration |
|---|---|
| Original language | Russian |
| Pages (from-to) | 28-32 |
| Number of pages | 5 |
| Journal | Ontogenez |
| Volume | 25 |
| Issue number | 6 |
| Publication status | Published - Nov 1994 |