TY - JOUR
T1 - Mechanism of uranium (VI) removal by two anaerobic bacterial communities
AU - Martins, Mónica
AU - Faleiro, Maria Leonor
AU - da Costa, Ana M.Rosa
AU - Chaves, Sandra
AU - Tenreiro, Rogério
AU - Matos, António Pedro
AU - Costa, Maria Clara
N1 - Funding Information:
Funding by Fundação para a Ciência e a Tecnologia through a PhD grant ( SFRH/BD/29677/2006 ) is acknowledged. Authors are grateful to Júlio César Hernández from Universidad de Huelva, Faculdad de Ciencias Experimentales, Spain, for the XRD analysis.
PY - 2010
Y1 - 2010
N2 - The mechanism of uranium (VI) removal by two anaerobic bacterial consortia, recovered from an uncontaminated site (consortium A) and other from an uranium mine (consortium U), was investigated. The highest efficiency of U (VI) removal by both consortia (97%) occurred at room temperature and at pH 7.2. Furthermore, it was found that U (VI) removal by consortium A occurred by enzymatic reduction and bioaccumulation, while the enzymatic process was the only mechanism involved in metal removal by consortium U. FTIR analysis suggested that after U (VI) reduction, U (IV) could be bound to carboxyl, phosphate and amide groups of bacterial cells. Phylogenetic analysis of 16S rRNA showed that community A was mainly composed by bacteria closely related to Sporotalea genus and Rhodocyclaceae family, while community U was mainly composed by bacteria related to Clostridium genus and Rhodocyclaceae family.
AB - The mechanism of uranium (VI) removal by two anaerobic bacterial consortia, recovered from an uncontaminated site (consortium A) and other from an uranium mine (consortium U), was investigated. The highest efficiency of U (VI) removal by both consortia (97%) occurred at room temperature and at pH 7.2. Furthermore, it was found that U (VI) removal by consortium A occurred by enzymatic reduction and bioaccumulation, while the enzymatic process was the only mechanism involved in metal removal by consortium U. FTIR analysis suggested that after U (VI) reduction, U (IV) could be bound to carboxyl, phosphate and amide groups of bacterial cells. Phylogenetic analysis of 16S rRNA showed that community A was mainly composed by bacteria closely related to Sporotalea genus and Rhodocyclaceae family, while community U was mainly composed by bacteria related to Clostridium genus and Rhodocyclaceae family.
KW - Bacterial consortia
KW - Bio-removal mechanism
KW - Bioremediation
KW - Uranium (VI)
UR - http://www.scopus.com/inward/record.url?scp=77957858726&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2010.08.009
DO - 10.1016/j.jhazmat.2010.08.009
M3 - Article
C2 - 20832165
AN - SCOPUS:77957858726
SN - 0304-3894
VL - 184
SP - 89
EP - 96
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
IS - 1-3
ER -
