Molecular construction of bionanoparticles: Chimaeric SIV p17-HIV 1 p6 nanoparticles with minimal viral protein content

Maria J.L. Costa; Luísa Pedro; António P.Alves De Matos; Maria R. Aires-Barros; José A. Belo; João Goncalves; Guilherme N.M. Ferreira

doi:10.1042/BA20060208

Molecular construction of bionanoparticles: Chimaeric SIV p17-HIV 1 p6 nanoparticles with minimal viral protein content

Maria J.L. Costa, Luísa Pedro, António P.Alves De Matos, Maria R. Aires-Barros, José A. Belo, João Goncalves, Guilherme N.M. Ferreira

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

VLPs (virus-like particles) are promising delivery vectors for molecular therapy, since they combine the major advantages of viral vectors with significantly fewer viral vector disadvantages. The present paper describes the molecular construction of chimaeric VLPs based on minimal SIV (simian immunodeficiency virus) and HIV1 components. A chimaeric protein was constructed by fusion of SIV matrix protein (p17) and HIV1 p6 protein, and we demonstrated that the chimaeric proteins assemble as 80 nm nanoparticles containing ∼7700 chimaeric protein units. Chimaeric VLPs are released from HEK-293T cells (human embryonic kidney cells expressing the large T-antigen of simian virus 40) and are fully encapsulated with lipid membrane. Chimaeric VLPs are produced at 3.7-fold higher levels when compared with SIV p17 VLPs owing to duplication of a PTAP (Pro-Thr-Ala-Pro) domain previously shown as essential for virus particle release. The chimaeric VLPs constructed in the present paper were efficiently pseudotyped with vesicular-stomatitis-virus glycoprotein, as shown by immunoprecipitation assays.

Original language	English
Pages (from-to)	35-43
Number of pages	9
Journal	Biotechnology and Applied Biochemistry
Volume	48
Issue number	1
DOIs	https://doi.org/10.1042/BA20060208
Publication status	Published - Sept 2007
Externally published	Yes

Keywords

Bionanoparticle
Delivery vector
Gene therapy
Human and simian immunodeficiency virus (HIV and SIV)
Molecular therapy
Virus-like particle

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Molecular construction of bionanoparticles: Chimaeric SIV p17-HIV 1 p6 nanoparticles with minimal viral protein content",

abstract = "VLPs (virus-like particles) are promising delivery vectors for molecular therapy, since they combine the major advantages of viral vectors with significantly fewer viral vector disadvantages. The present paper describes the molecular construction of chimaeric VLPs based on minimal SIV (simian immunodeficiency virus) and HIV1 components. A chimaeric protein was constructed by fusion of SIV matrix protein (p17) and HIV1 p6 protein, and we demonstrated that the chimaeric proteins assemble as 80 nm nanoparticles containing ∼7700 chimaeric protein units. Chimaeric VLPs are released from HEK-293T cells (human embryonic kidney cells expressing the large T-antigen of simian virus 40) and are fully encapsulated with lipid membrane. Chimaeric VLPs are produced at 3.7-fold higher levels when compared with SIV p17 VLPs owing to duplication of a PTAP (Pro-Thr-Ala-Pro) domain previously shown as essential for virus particle release. The chimaeric VLPs constructed in the present paper were efficiently pseudotyped with vesicular-stomatitis-virus glycoprotein, as shown by immunoprecipitation assays.",

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author = "Costa, {Maria J.L.} and Lu{\'i}sa Pedro and {De Matos}, {Ant{\'o}nio P.Alves} and Aires-Barros, {Maria R.} and Belo, {Jos{\'e} A.} and Jo{\~a}o Goncalves and Ferreira, {Guilherme N.M.}",

year = "2007",

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T2 - Chimaeric SIV p17-HIV 1 p6 nanoparticles with minimal viral protein content

AU - Costa, Maria J.L.

AU - Pedro, Luísa

AU - De Matos, António P.Alves

AU - Aires-Barros, Maria R.

AU - Belo, José A.

AU - Goncalves, João

AU - Ferreira, Guilherme N.M.

PY - 2007/9

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N2 - VLPs (virus-like particles) are promising delivery vectors for molecular therapy, since they combine the major advantages of viral vectors with significantly fewer viral vector disadvantages. The present paper describes the molecular construction of chimaeric VLPs based on minimal SIV (simian immunodeficiency virus) and HIV1 components. A chimaeric protein was constructed by fusion of SIV matrix protein (p17) and HIV1 p6 protein, and we demonstrated that the chimaeric proteins assemble as 80 nm nanoparticles containing ∼7700 chimaeric protein units. Chimaeric VLPs are released from HEK-293T cells (human embryonic kidney cells expressing the large T-antigen of simian virus 40) and are fully encapsulated with lipid membrane. Chimaeric VLPs are produced at 3.7-fold higher levels when compared with SIV p17 VLPs owing to duplication of a PTAP (Pro-Thr-Ala-Pro) domain previously shown as essential for virus particle release. The chimaeric VLPs constructed in the present paper were efficiently pseudotyped with vesicular-stomatitis-virus glycoprotein, as shown by immunoprecipitation assays.

AB - VLPs (virus-like particles) are promising delivery vectors for molecular therapy, since they combine the major advantages of viral vectors with significantly fewer viral vector disadvantages. The present paper describes the molecular construction of chimaeric VLPs based on minimal SIV (simian immunodeficiency virus) and HIV1 components. A chimaeric protein was constructed by fusion of SIV matrix protein (p17) and HIV1 p6 protein, and we demonstrated that the chimaeric proteins assemble as 80 nm nanoparticles containing ∼7700 chimaeric protein units. Chimaeric VLPs are released from HEK-293T cells (human embryonic kidney cells expressing the large T-antigen of simian virus 40) and are fully encapsulated with lipid membrane. Chimaeric VLPs are produced at 3.7-fold higher levels when compared with SIV p17 VLPs owing to duplication of a PTAP (Pro-Thr-Ala-Pro) domain previously shown as essential for virus particle release. The chimaeric VLPs constructed in the present paper were efficiently pseudotyped with vesicular-stomatitis-virus glycoprotein, as shown by immunoprecipitation assays.

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KW - Delivery vector

KW - Gene therapy

KW - Human and simian immunodeficiency virus (HIV and SIV)

KW - Molecular therapy

KW - Virus-like particle

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Molecular construction of bionanoparticles: Chimaeric SIV p17-HIV 1 p6 nanoparticles with minimal viral protein content

Abstract

Keywords

UN SDGs

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