TY - GEN
T1 - Nitrite Biosensing Using Cytochrome C Nitrite Reductase
T2 - 5th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2012
AU - Correia, Cátia
AU - Rodrigues, Marcelo
AU - Silveira, Célia M.
AU - Moura, José J.G.
AU - Ochoteco, Estibaliz
AU - Jubete, Elena
AU - Almeida, M. Gabriela
PY - 2013
Y1 - 2013
N2 - This paper presents the results of a primary study that aims to produce miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, here we aimed at reducing the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with the ccNiR enzyme, previously mixed with the conductive graphite ink. Firstly, though, the electrode preparation was optimized (enzyme amount, organic solvent and curing temperature). Then, the biocompatibility of ccNiR with these harsh treatments and the analytical performance of the modified electrodes were evaluated by cyclic voltammetry. Finally, the carbon paste screen-printed electrodes were coated with the ccNiR/carbon ink composite, displaying a good sensitivity (5.3x10- 7 A.uM- 1.cm- 2) within the linear range of 0.001 - 1.5 mM.
AB - This paper presents the results of a primary study that aims to produce miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, here we aimed at reducing the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with the ccNiR enzyme, previously mixed with the conductive graphite ink. Firstly, though, the electrode preparation was optimized (enzyme amount, organic solvent and curing temperature). Then, the biocompatibility of ccNiR with these harsh treatments and the analytical performance of the modified electrodes were evaluated by cyclic voltammetry. Finally, the carbon paste screen-printed electrodes were coated with the ccNiR/carbon ink composite, displaying a good sensitivity (5.3x10- 7 A.uM- 1.cm- 2) within the linear range of 0.001 - 1.5 mM.
KW - Cytochrome c nitrite reductase
KW - Electrochemical biosensors
KW - Nitrite
KW - Screen printed electrodes
UR - http://www.scopus.com/inward/record.url?scp=84904642582&partnerID=8YFLogxK
U2 - 10.1007/978-3-642-38256-7_3
DO - 10.1007/978-3-642-38256-7_3
M3 - Conference contribution
AN - SCOPUS:84904642582
SN - 9783642382550
T3 - Communications in Computer and Information Science
SP - 41
EP - 50
BT - Biomedical Engineering Systems and Technologies - 5th International Joint Conference, BIOSTEC 2012, Revised Selected Papers
PB - Springer Verlag
Y2 - 1 February 2012 through 4 February 2012
ER -