TY - GEN
T1 - Nitrite biosensing with disposable electrode strips
T2 - International Conference on Biomedical Electronics and Devices, BIODEVICES 2012
AU - Rodrigues, Marcelo
AU - Correia, Cátia
AU - Silveira, Célia M.
AU - Moura, José J.G.
AU - Ochoteco, Estibaliz
AU - Jubete, Elena
AU - Almeida, M. Gabriela
PY - 2012
Y1 - 2012
N2 - This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40°C or 60°C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performance of these electrodes was satisfactory, with a sensitivity of 52 A.μM -1.cm -2 within a linear range of 0.001 - 1 mM.
AB - This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40°C or 60°C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performance of these electrodes was satisfactory, with a sensitivity of 52 A.μM -1.cm -2 within a linear range of 0.001 - 1 mM.
KW - Cytochrome c nitrite reductase
KW - Electrochemical biosensors
KW - Nitrite
KW - Screen printed electrodes
UR - http://www.scopus.com/inward/record.url?scp=84861967204&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84861967204
SN - 9789898425911
T3 - BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices
SP - 32
EP - 36
BT - BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices
Y2 - 1 February 2012 through 4 February 2012
ER -