TY - JOUR
T1 - Poly(3-hydroxybutyrate) production by Cupriavidus necator using waste glycerol
AU - Cavalheiro, João M.B.T.
AU - de Almeida, M. Catarina M.D.
AU - Grandfils, Christian
AU - da Fonseca, M. M.R.
N1 - Funding Information:
This work is being financed by the EU Integrated Project BIOPRODUCTION (contract no. 026515-2). Catarina Almeida is supported by a fellowship from Fundação para a Ciência e Tecnologia, Portugal (SFRH/BPD/26678/2006). We are thankful to Dr. P. Leitão, Oleocom, SA, Portugal, for the GRP supply.
PY - 2009/5
Y1 - 2009/5
N2 - Polyhydroxyalkanoates (PHAs) have been recognized as good substitutes for the non-biodegradable petrochemically produced polymers. However, their high (real or estimated) current production cost limits their industrial applications. This work exploits two strategies to enhance PHAs substitution potential: the increase in PHA volumetric productivity in high density cultures and the use of waste glycerol (GRP), a by-product from the biodiesel industry, as primary carbon source for cell growth and polymer synthesis. Cupriavidus necator DSM 545 was used to accumulate poly(3-hydroxybutyrate) (P(3HB)) from GRP and from commercial glycerol (PG) as control substrate. On PG, productivities between 0.6 gPHB L-1 h-1 and 1.5 gPHB L-1 h-1 were attained. The maximum cell DW was 82.5 gDW L-1, the P(3HB) content being 62%. When GRP was used, 68.8 gDW L-1 with a P(3HB) accumulation of 38% resulting in a final productivity of 0.84 gPHB L-1 h-1 was obtained. By decreasing the biomass concentration at which accumulation was triggered, a productivity of 1.1 gPHB L-1 h-1 (50% P(3HB), w/w) was attained using GRP. P(3HB) molecular weights (Mw) ranged from 7.9 × 105 to 9.6 × 105 Da.
AB - Polyhydroxyalkanoates (PHAs) have been recognized as good substitutes for the non-biodegradable petrochemically produced polymers. However, their high (real or estimated) current production cost limits their industrial applications. This work exploits two strategies to enhance PHAs substitution potential: the increase in PHA volumetric productivity in high density cultures and the use of waste glycerol (GRP), a by-product from the biodiesel industry, as primary carbon source for cell growth and polymer synthesis. Cupriavidus necator DSM 545 was used to accumulate poly(3-hydroxybutyrate) (P(3HB)) from GRP and from commercial glycerol (PG) as control substrate. On PG, productivities between 0.6 gPHB L-1 h-1 and 1.5 gPHB L-1 h-1 were attained. The maximum cell DW was 82.5 gDW L-1, the P(3HB) content being 62%. When GRP was used, 68.8 gDW L-1 with a P(3HB) accumulation of 38% resulting in a final productivity of 0.84 gPHB L-1 h-1 was obtained. By decreasing the biomass concentration at which accumulation was triggered, a productivity of 1.1 gPHB L-1 h-1 (50% P(3HB), w/w) was attained using GRP. P(3HB) molecular weights (Mw) ranged from 7.9 × 105 to 9.6 × 105 Da.
KW - Biodiesel
KW - Cupriavidus necator DSM 545
KW - Fermentation
KW - Glycerol
KW - Poly(3-hydroxybutyrate)
KW - Waste GRP
UR - http://www.scopus.com/inward/record.url?scp=64449088957&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2009.01.008
DO - 10.1016/j.procbio.2009.01.008
M3 - Article
AN - SCOPUS:64449088957
SN - 1359-5113
VL - 44
SP - 509
EP - 515
JO - Process Biochemistry
JF - Process Biochemistry
IS - 5
ER -
