Polydioxanone powder induces collagen synthesis in fibroblast culture? – A pilot study

Background: Polydioxanone powder appears in the scenario of biostimulators as an effective and minimally invasive alternative to delay the effects of aging on the skin caused by the degradation of collagen and elastic fibers. Objectives: To verify, in vitro, the ability of a polydioxanone powder to induce collagen production in a culture of human fibroblasts and compare the results with the induction promoted by the PLLA powder. Methods: The study evaluated if CCD-1072Sk human fibroblasts in adherent cultures (ATCC CRL-2088), into eight groups (n=12) C, PLLA, PDO, PDO^# organized into two subgroups PLATES A (24h) AND PLATES B (48h) if Poly-L-lactic acid-PLLA (Rennova® elleva) and powdered polydioxanone-PDO (UltraCol – Ultra-V® Medical) would stimulate an increase in collagen production by fibroblasts. The Shap-iro-Wilk test was used to analyze the data’s normality. To analyze the effect of the time in which the samples were analyzed (24h or 48h) and of the C, PLLA, PDO, PDO^# group on collagen concentrations, we used the Two-Way ANOVA test. To identify significant differences between subgroups, we used Tukey ‘s post-hoc. Results: Variables are presented as mean and standard deviation (SD). The results showed a significant increase in collagen production from 24h to 48h in the four analyzed groups C, PLLA, PDO and PDO^#. Conclusion: None of the biostimulators positively influenced the production of collage.