TY - JOUR
T1 - Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2
T2 - Identification of a new antigenic epitope
AU - Marcelino, J. M.
AU - Novo, C.
AU - Pereira, J. M.
AU - Picotez, F.
AU - Clemente, A.
AU - Taveira, N.
PY - 2001
Y1 - 2001
N2 - One anticapsid (p26) mouse monoclonal antibody was developed after immunization with recombinant p26 Gag protein and was tested for reactivity with different HIV-1 and HIV-2 isolates by ELISA and Western blot analysis. This antibody, named R26.1, reacted with all HIV-2 isolates tested and with recombinant p26 proteins, but no HIV-1 isolates. The epitope of antibody R26.1 was mapped to residues 50-71 in the N-terminal domain of the capsid protein, a highly conserved region in all HIV-2 isolates sequenced to date. This monoclonal antibody may be useful for the detection of HIV-2, and for the discrimination between HIV-1 and HIV-2 infections. Likewise, the identified epitope may be useful for the detection of p26 antibodies in HIV-2-infected individuals.
AB - One anticapsid (p26) mouse monoclonal antibody was developed after immunization with recombinant p26 Gag protein and was tested for reactivity with different HIV-1 and HIV-2 isolates by ELISA and Western blot analysis. This antibody, named R26.1, reacted with all HIV-2 isolates tested and with recombinant p26 proteins, but no HIV-1 isolates. The epitope of antibody R26.1 was mapped to residues 50-71 in the N-terminal domain of the capsid protein, a highly conserved region in all HIV-2 isolates sequenced to date. This monoclonal antibody may be useful for the detection of HIV-2, and for the discrimination between HIV-1 and HIV-2 infections. Likewise, the identified epitope may be useful for the detection of p26 antibodies in HIV-2-infected individuals.
UR - http://www.scopus.com/inward/record.url?scp=0034803490&partnerID=8YFLogxK
U2 - 10.1089/088922201750461348
DO - 10.1089/088922201750461348
M3 - Article
C2 - 11559428
AN - SCOPUS:0034803490
SN - 0889-2229
VL - 17
SP - 1279
EP - 1283
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
IS - 13
ER -