Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: Identification of a new antigenic epitope

J. M. Marcelino, C. Novo, J. M. Pereira, F. Picotez, A. Clemente, N. Taveira

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Abstract

One anticapsid (p26) mouse monoclonal antibody was developed after immunization with recombinant p26 Gag protein and was tested for reactivity with different HIV-1 and HIV-2 isolates by ELISA and Western blot analysis. This antibody, named R26.1, reacted with all HIV-2 isolates tested and with recombinant p26 proteins, but no HIV-1 isolates. The epitope of antibody R26.1 was mapped to residues 50-71 in the N-terminal domain of the capsid protein, a highly conserved region in all HIV-2 isolates sequenced to date. This monoclonal antibody may be useful for the detection of HIV-2, and for the discrimination between HIV-1 and HIV-2 infections. Likewise, the identified epitope may be useful for the detection of p26 antibodies in HIV-2-infected individuals.

Original languageEnglish
Pages (from-to)1279-1283
Number of pages5
JournalAIDS Research and Human Retroviruses
Volume17
Issue number13
DOIs
Publication statusPublished - 2001
Externally publishedYes

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