Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: Identification of a new antigenic epitope

J. M. Marcelino; C. Novo; J. M. Pereira; F. Picotez; A. Clemente; N. Taveira

doi:10.1089/088922201750461348

Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: Identification of a new antigenic epitope

J. M. Marcelino
, C. Novo
, J. M. Pereira
, F. Picotez
, A. Clemente
, N. Taveira

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

One anticapsid (p26) mouse monoclonal antibody was developed after immunization with recombinant p26 Gag protein and was tested for reactivity with different HIV-1 and HIV-2 isolates by ELISA and Western blot analysis. This antibody, named R26.1, reacted with all HIV-2 isolates tested and with recombinant p26 proteins, but no HIV-1 isolates. The epitope of antibody R26.1 was mapped to residues 50-71 in the N-terminal domain of the capsid protein, a highly conserved region in all HIV-2 isolates sequenced to date. This monoclonal antibody may be useful for the detection of HIV-2, and for the discrimination between HIV-1 and HIV-2 infections. Likewise, the identified epitope may be useful for the detection of p26 antibodies in HIV-2-infected individuals.

Original language	English
Pages (from-to)	1279-1283
Number of pages	5
Journal	AIDS Research and Human Retroviruses
Volume	17
Issue number	13
DOIs	https://doi.org/10.1089/088922201750461348
Publication status	Published - 2001
Externally published	Yes

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title = "Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: Identification of a new antigenic epitope",

abstract = "One anticapsid (p26) mouse monoclonal antibody was developed after immunization with recombinant p26 Gag protein and was tested for reactivity with different HIV-1 and HIV-2 isolates by ELISA and Western blot analysis. This antibody, named R26.1, reacted with all HIV-2 isolates tested and with recombinant p26 proteins, but no HIV-1 isolates. The epitope of antibody R26.1 was mapped to residues 50-71 in the N-terminal domain of the capsid protein, a highly conserved region in all HIV-2 isolates sequenced to date. This monoclonal antibody may be useful for the detection of HIV-2, and for the discrimination between HIV-1 and HIV-2 infections. Likewise, the identified epitope may be useful for the detection of p26 antibodies in HIV-2-infected individuals.",

author = "Marcelino, \{J. M.\} and C. Novo and Pereira, \{J. M.\} and F. Picotez and A. Clemente and N. Taveira",

year = "2001",

doi = "10.1089/088922201750461348",

language = "English",

volume = "17",

pages = "1279--1283",

journal = "AIDS Research and Human Retroviruses",

issn = "0889-2229",

publisher = "Mary Ann Liebert Inc.",

number = "13",

}

Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: Identification of a new antigenic epitope. / Marcelino, J. M.; Novo, C.; Pereira, J. M. et al.
In: AIDS Research and Human Retroviruses, Vol. 17, No. 13, 2001, p. 1279-1283.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2

T2 - Identification of a new antigenic epitope

AU - Marcelino, J. M.

AU - Novo, C.

AU - Pereira, J. M.

AU - Picotez, F.

AU - Clemente, A.

AU - Taveira, N.

PY - 2001

Y1 - 2001

N2 - One anticapsid (p26) mouse monoclonal antibody was developed after immunization with recombinant p26 Gag protein and was tested for reactivity with different HIV-1 and HIV-2 isolates by ELISA and Western blot analysis. This antibody, named R26.1, reacted with all HIV-2 isolates tested and with recombinant p26 proteins, but no HIV-1 isolates. The epitope of antibody R26.1 was mapped to residues 50-71 in the N-terminal domain of the capsid protein, a highly conserved region in all HIV-2 isolates sequenced to date. This monoclonal antibody may be useful for the detection of HIV-2, and for the discrimination between HIV-1 and HIV-2 infections. Likewise, the identified epitope may be useful for the detection of p26 antibodies in HIV-2-infected individuals.

AB - One anticapsid (p26) mouse monoclonal antibody was developed after immunization with recombinant p26 Gag protein and was tested for reactivity with different HIV-1 and HIV-2 isolates by ELISA and Western blot analysis. This antibody, named R26.1, reacted with all HIV-2 isolates tested and with recombinant p26 proteins, but no HIV-1 isolates. The epitope of antibody R26.1 was mapped to residues 50-71 in the N-terminal domain of the capsid protein, a highly conserved region in all HIV-2 isolates sequenced to date. This monoclonal antibody may be useful for the detection of HIV-2, and for the discrimination between HIV-1 and HIV-2 infections. Likewise, the identified epitope may be useful for the detection of p26 antibodies in HIV-2-infected individuals.

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U2 - 10.1089/088922201750461348

DO - 10.1089/088922201750461348

M3 - Article

C2 - 11559428

AN - SCOPUS:0034803490

SN - 0889-2229

VL - 17

SP - 1279

EP - 1283

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

IS - 13

ER -

Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: Identification of a new antigenic epitope

Abstract

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