Production of biopolyhydroxyalkanoates

Catarina Dias De Almeida; Maria Teresa Cesario; Manuela Regalo Da Fonseca

doi:10.1109/ENBENG.2011.6026078

Production of biopolyhydroxyalkanoates

Catarina Dias De Almeida, Maria Teresa Cesario, Manuela Regalo Da Fonseca

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

This work focuses on developing high productivity and economically viable processes for the production of biopolyesters with potential uses in biomedical fields. High cell density fed-batch cultures of Cupriavidus necator on waste glycerol were used to produce the copolymer P(3HB-co-4HB). The incorporation of 4HB monomers within the polymeric chain was achieved through the concomitant feeding of gamma-butyrolactone (GBL). Acetic and propionic acid were tested as stimulators to enhance 4HB incorporation. When propionic acid was used, a terpolymer P(3HB-co-4HB-co-3HV) was originated. P(3HB-co-4HB) copolymers with molar % of 4HB from 1.8 % to 12.3 % were produced with productivities between 0.09 g_P(3HB-4HB). L¹.h¹ and 0.79 g _P(3HB-4HB) L¹.h¹.

Original language	English
Title of host publication	1st Portuguese Meeting in Biomedical Engineering, ENBENG 2011
DOIs	https://doi.org/10.1109/ENBENG.2011.6026078
Publication status	Published - 2011
Event	2011 1st Portuguese Meeting in Bioengineering: The Challenge of the XXI Century, ENBENG 2011 - Lisbon, Portugal Duration: 1 Mar 2011 → 4 Mar 2011

Publication series

Name	1st Portuguese Meeting in Biomedical Engineering, ENBENG 2011

Conference

Conference	2011 1st Portuguese Meeting in Bioengineering: The Challenge of the XXI Century, ENBENG 2011
Country/Territory	Portugal
City	Lisbon
Period	1/03/11 → 4/03/11

Keywords

Cupriavidus necator DSM 545
P(3HB-co-4HB)
PHA
biopolyesters
waste glycerol

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10.1109/ENBENG.2011.6026078

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title = "Production of biopolyhydroxyalkanoates",

abstract = "This work focuses on developing high productivity and economically viable processes for the production of biopolyesters with potential uses in biomedical fields. High cell density fed-batch cultures of Cupriavidus necator on waste glycerol were used to produce the copolymer P(3HB-co-4HB). The incorporation of 4HB monomers within the polymeric chain was achieved through the concomitant feeding of gamma-butyrolactone (GBL). Acetic and propionic acid were tested as stimulators to enhance 4HB incorporation. When propionic acid was used, a terpolymer P(3HB-co-4HB-co-3HV) was originated. P(3HB-co-4HB) copolymers with molar % of 4HB from 1.8 % to 12.3 % were produced with productivities between 0.09 gP(3HB-4HB). L1.h1 and 0.79 g P(3HB-4HB) L1.h1.",

keywords = "Cupriavidus necator DSM 545, P(3HB-co-4HB), PHA, biopolyesters, waste glycerol",

author = "{De Almeida}, {Catarina Dias} and Cesario, {Maria Teresa} and {Da Fonseca}, {Manuela Regalo}",

year = "2011",

doi = "10.1109/ENBENG.2011.6026078",

language = "English",

isbn = "9781457705229",

series = "1st Portuguese Meeting in Biomedical Engineering, ENBENG 2011",

booktitle = "1st Portuguese Meeting in Biomedical Engineering, ENBENG 2011",

note = "2011 1st Portuguese Meeting in Bioengineering: The Challenge of the XXI Century, ENBENG 2011 ; Conference date: 01-03-2011 Through 04-03-2011",

}

De Almeida, CD, Cesario, MT & Da Fonseca, MR 2011, Production of biopolyhydroxyalkanoates. in 1st Portuguese Meeting in Biomedical Engineering, ENBENG 2011., 6026078, 1st Portuguese Meeting in Biomedical Engineering, ENBENG 2011, 2011 1st Portuguese Meeting in Bioengineering: The Challenge of the XXI Century, ENBENG 2011, Lisbon, Portugal, 1/03/11. https://doi.org/10.1109/ENBENG.2011.6026078

TY - GEN

T1 - Production of biopolyhydroxyalkanoates

AU - De Almeida, Catarina Dias

AU - Cesario, Maria Teresa

AU - Da Fonseca, Manuela Regalo

PY - 2011

Y1 - 2011

N2 - This work focuses on developing high productivity and economically viable processes for the production of biopolyesters with potential uses in biomedical fields. High cell density fed-batch cultures of Cupriavidus necator on waste glycerol were used to produce the copolymer P(3HB-co-4HB). The incorporation of 4HB monomers within the polymeric chain was achieved through the concomitant feeding of gamma-butyrolactone (GBL). Acetic and propionic acid were tested as stimulators to enhance 4HB incorporation. When propionic acid was used, a terpolymer P(3HB-co-4HB-co-3HV) was originated. P(3HB-co-4HB) copolymers with molar % of 4HB from 1.8 % to 12.3 % were produced with productivities between 0.09 gP(3HB-4HB). L1.h1 and 0.79 g P(3HB-4HB) L1.h1.

AB - This work focuses on developing high productivity and economically viable processes for the production of biopolyesters with potential uses in biomedical fields. High cell density fed-batch cultures of Cupriavidus necator on waste glycerol were used to produce the copolymer P(3HB-co-4HB). The incorporation of 4HB monomers within the polymeric chain was achieved through the concomitant feeding of gamma-butyrolactone (GBL). Acetic and propionic acid were tested as stimulators to enhance 4HB incorporation. When propionic acid was used, a terpolymer P(3HB-co-4HB-co-3HV) was originated. P(3HB-co-4HB) copolymers with molar % of 4HB from 1.8 % to 12.3 % were produced with productivities between 0.09 gP(3HB-4HB). L1.h1 and 0.79 g P(3HB-4HB) L1.h1.

KW - Cupriavidus necator DSM 545

KW - P(3HB-co-4HB)

KW - PHA

KW - biopolyesters

KW - waste glycerol

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U2 - 10.1109/ENBENG.2011.6026078

DO - 10.1109/ENBENG.2011.6026078

M3 - Conference contribution

AN - SCOPUS:80053980609

SN - 9781457705229

T3 - 1st Portuguese Meeting in Biomedical Engineering, ENBENG 2011

BT - 1st Portuguese Meeting in Biomedical Engineering, ENBENG 2011

T2 - 2011 1st Portuguese Meeting in Bioengineering: The Challenge of the XXI Century, ENBENG 2011

Y2 - 1 March 2011 through 4 March 2011

ER -

Production of biopolyhydroxyalkanoates

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