TY - JOUR
T1 - Protein Synthesis Directed by Polyadenylated and Non‐polyadenylated RNA
T2 - Isolated from Membrane‐Bound and Free Polysomes of Tetrahymena pyriformis
AU - BARAHONA, Isabel
AU - GALEGO, Lisete
AU - RODRIGUES‐POUSADA, Claudina
PY - 1983/3
Y1 - 1983/3
N2 - Free and membrane‐bound polysomes were prepared from the protozoa Tetrahymena pyriformis using a procedure which gives good recovery and practically no cross‐contamination. Polysomes are intact as analysed by sedimentation analysis. Poly(A)‐rich RNA and poly(A)‐free RNA, isolated from both populations of polysomes, show similar electrophoretic patterns. These RNAs were translated in the rabbit reticulocyte lysate cell‐free system and the translation products were analysed by one‐dimensional and two‐dimensional gel electrophoresis. The most striking differences were found in the two‐dimensional electrophoretic analysis namely: (a) a group of polypeptides (10) is synthesized mainly on membrane‐bound polysomes, (b) a second abundant group is synthesized mainly in free polysomes (c) and a third class of polypeptides is synthesized on both kinds of polysomes. Poly(A)‐free RNAs, isolated from free polysomes, are also able to promote synthesis of some polypeptides. The results are discussed taking into account the fact that T. pyriformis is a non‐secretory cell.
AB - Free and membrane‐bound polysomes were prepared from the protozoa Tetrahymena pyriformis using a procedure which gives good recovery and practically no cross‐contamination. Polysomes are intact as analysed by sedimentation analysis. Poly(A)‐rich RNA and poly(A)‐free RNA, isolated from both populations of polysomes, show similar electrophoretic patterns. These RNAs were translated in the rabbit reticulocyte lysate cell‐free system and the translation products were analysed by one‐dimensional and two‐dimensional gel electrophoresis. The most striking differences were found in the two‐dimensional electrophoretic analysis namely: (a) a group of polypeptides (10) is synthesized mainly on membrane‐bound polysomes, (b) a second abundant group is synthesized mainly in free polysomes (c) and a third class of polypeptides is synthesized on both kinds of polysomes. Poly(A)‐free RNAs, isolated from free polysomes, are also able to promote synthesis of some polypeptides. The results are discussed taking into account the fact that T. pyriformis is a non‐secretory cell.
UR - http://www.scopus.com/inward/record.url?scp=0020724041&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1983.tb07245.x
DO - 10.1111/j.1432-1033.1983.tb07245.x
M3 - Article
C2 - 6187567
AN - SCOPUS:0020724041
SN - 0014-2956
VL - 131
SP - 171
EP - 176
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 1
ER -