TY - JOUR
T1 - Protein synthesis in ovine muscle and skin
T2 - Sequential measurements with three different amino acids based on the large-dose procedure
AU - Rocha, Humberto J.G.
AU - Nash, Joanna E.
AU - Connell, Alexmary
AU - Lobley, Gerald E.
N1 - Funding Information:
Acknowledgements--The invaluable assistance of Mrs V. Buchan for amino acid separations and analysis and Messrs E. Milne and G. Calder for mass spectrometric analysis is gratefully acknowledged. This work was funded by the Scottish Orifice Agriculture and Fisheries Department as part of the core budget to the Rowett Research Institute. HJGR and JN are recipients of student awards by the European Community and the Ministry of Agriculture, Fisheries and Food, respectively.
PY - 1993/6
Y1 - 1993/6
N2 - 1. 1. Protein synthesis has been determined in biopsies from ovine skin and muscle by sequential use of three [13C] amino acids, valine leucine and phenylalanine, as large-dose injections. 2. 2. Leucine and phenylalanine increased plasma insulin concentrations within 40 min of injection. 3. 3. All three amino acids decreased the plasma concentrations of other amino acids. 4. 4. Intracellular free amino acids in muscle decreased while those in skin increased. 5. 5. The fractional rates of protein synthesis were similar, regardless of which amino acid was used, although the rates for muscle were significantly less than for skin (2.1 vs 11.0%/day, P < 0.001).
AB - 1. 1. Protein synthesis has been determined in biopsies from ovine skin and muscle by sequential use of three [13C] amino acids, valine leucine and phenylalanine, as large-dose injections. 2. 2. Leucine and phenylalanine increased plasma insulin concentrations within 40 min of injection. 3. 3. All three amino acids decreased the plasma concentrations of other amino acids. 4. 4. Intracellular free amino acids in muscle decreased while those in skin increased. 5. 5. The fractional rates of protein synthesis were similar, regardless of which amino acid was used, although the rates for muscle were significantly less than for skin (2.1 vs 11.0%/day, P < 0.001).
UR - http://www.scopus.com/inward/record.url?scp=0027196483&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(93)90233-U
DO - 10.1016/0305-0491(93)90233-U
M3 - Article
C2 - 8359018
AN - SCOPUS:0027196483
SN - 0305-0491
VL - 105
SP - 301
EP - 307
JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
IS - 2
ER -