TY - JOUR
T1 - Quantitation of human immunodeficiency virus type 2 DNA in peripheral blood mononuclear cells by using a quantitative-competitive PCR assay
AU - Gomes, Perpétua
AU - Taveira, Nuno Costa
AU - Moniz Pereira, José
AU - Antunes, Francisco
AU - Santos Ferreira, M. O.
AU - Lourenço, Maria Helena
PY - 1999
Y1 - 1999
N2 - A new quantitative-competitive PCR-based human immunodeficiency virus type 2 (HIV-2) proviral DNA assay (QC-PCR) was developed and used to determine the provital load in HIV-2-infected individuals. Proviral load varied considerably, with means of 1,831 copies per 106 peripheral blood mononuclear cells for asymptomatic subjects (n = 19) and 2,587 for AIDS patients (n = 2). HIV-2 viral and provital loads also varied significantly over time in asymptomatic patients. These data suggest that a high level of virus replication occurs throughout the asymptomatic phase of HIV-2 infection.
AB - A new quantitative-competitive PCR-based human immunodeficiency virus type 2 (HIV-2) proviral DNA assay (QC-PCR) was developed and used to determine the provital load in HIV-2-infected individuals. Proviral load varied considerably, with means of 1,831 copies per 106 peripheral blood mononuclear cells for asymptomatic subjects (n = 19) and 2,587 for AIDS patients (n = 2). HIV-2 viral and provital loads also varied significantly over time in asymptomatic patients. These data suggest that a high level of virus replication occurs throughout the asymptomatic phase of HIV-2 infection.
UR - http://www.scopus.com/inward/record.url?scp=0345683492&partnerID=8YFLogxK
U2 - 10.1128/jcm.37.2.453-456.1999
DO - 10.1128/jcm.37.2.453-456.1999
M3 - Article
C2 - 9889242
AN - SCOPUS:0345683492
SN - 0095-1137
VL - 37
SP - 453
EP - 456
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 2
ER -
