Quaternized cashew gum: An anti-staphylococcal and biocompatible cationic polymer for biotechnological applications

Patrick V. Quelemes; Alyne R. de Araújo; Alexandra Plácido; Cristina Delerue-Matos; Jeanny S. Maciel; Lucinda J. Bessa; Alicia S. Ombredane; Graziella A. Joanitti; Maria José dos S. Soares; Peter Eaton; Durcilene A. da Silva; José Roberto S.A. Leite

doi:10.1016/j.carbpol.2016.10.026

Quaternized cashew gum: An anti-staphylococcal and biocompatible cationic polymer for biotechnological applications

Patrick V. Quelemes, Alyne R. de Araújo, Alexandra Plácido, Cristina Delerue-Matos, Jeanny S. Maciel, Lucinda J. Bessa, Alicia S. Ombredane, Graziella A. Joanitti, Maria José dos S. Soares, Peter Eaton, Durcilene A. da Silva, José Roberto S.A. Leite

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

Chemical modifications to cashew gum (CG) structure have been previously reported to obtain new physicochemical characteristics, however until now there were no reports of modifications by introduction of new functional groups to add cationic character. This study presents a quaternization route for CG using a quaternary ammonium reagent. The chemical features of the quaternized cashew gum derivatives (QCG) were analyzed by: FTIR, elemental analysis, degree of substitution, Zeta potential, ¹H NMR and ¹H-¹³C correlation (HSQC). QCG were evaluated for their anti-staphylococcal activity by determining minimum inhibitory and bactericidal concentrations against pathogenic Staphylococcus spp. and by imaging using atomic force microscopy. Moreover, the mammalian cell biocompatibility were also assessed through hemolytic and cell toxicity assays. QCG presented promising antimicrobial activity against methicillin-resistant S. aureus and biocompatibility on tested cells. These results show that QCG could be a promising tool in the development of biomaterials with an anti-septic action.

Original language	English
Pages (from-to)	567-575
Number of pages	9
Journal	Carbohydrate Polymers
Volume	157
DOIs	https://doi.org/10.1016/j.carbpol.2016.10.026
Publication status	Published - 10 Feb 2017
Externally published	Yes

Keywords

Antibacterial
Biocompatibility
Cashew gum
Quaternization
Staphylococcus spp.

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10.1016/j.carbpol.2016.10.026

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Quelemes, P. V., de Araújo, A. R., Plácido, A., Delerue-Matos, C., Maciel, J. S., Bessa, L. J., Ombredane, A. S., Joanitti, G. A., Soares, M. J. D. S., Eaton, P., da Silva, D. A., & Leite, J. R. S. A. (2017). Quaternized cashew gum: An anti-staphylococcal and biocompatible cationic polymer for biotechnological applications. Carbohydrate Polymers, 157, 567-575. https://doi.org/10.1016/j.carbpol.2016.10.026

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title = "Quaternized cashew gum: An anti-staphylococcal and biocompatible cationic polymer for biotechnological applications",

abstract = "Chemical modifications to cashew gum (CG) structure have been previously reported to obtain new physicochemical characteristics, however until now there were no reports of modifications by introduction of new functional groups to add cationic character. This study presents a quaternization route for CG using a quaternary ammonium reagent. The chemical features of the quaternized cashew gum derivatives (QCG) were analyzed by: FTIR, elemental analysis, degree of substitution, Zeta potential, 1H NMR and 1H-13C correlation (HSQC). QCG were evaluated for their anti-staphylococcal activity by determining minimum inhibitory and bactericidal concentrations against pathogenic Staphylococcus spp. and by imaging using atomic force microscopy. Moreover, the mammalian cell biocompatibility were also assessed through hemolytic and cell toxicity assays. QCG presented promising antimicrobial activity against methicillin-resistant S. aureus and biocompatibility on tested cells. These results show that QCG could be a promising tool in the development of biomaterials with an anti-septic action.",

keywords = "Antibacterial, Biocompatibility, Cashew gum, Quaternization, Staphylococcus spp.",

author = "Quelemes, {Patrick V.} and {de Ara{\'u}jo}, {Alyne R.} and Alexandra Pl{\'a}cido and Cristina Delerue-Matos and Maciel, {Jeanny S.} and Bessa, {Lucinda J.} and Ombredane, {Alicia S.} and Joanitti, {Graziella A.} and Soares, {Maria Jos{\'e} dos S.} and Peter Eaton and {da Silva}, {Durcilene A.} and Leite, {Jos{\'e} Roberto S.A.}",

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year = "2017",

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day = "10",

doi = "10.1016/j.carbpol.2016.10.026",

language = "English",

volume = "157",

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Quelemes, PV, de Araújo, AR, Plácido, A, Delerue-Matos, C, Maciel, JS, Bessa, LJ, Ombredane, AS, Joanitti, GA, Soares, MJDS, Eaton, P, da Silva, DA & Leite, JRSA 2017, 'Quaternized cashew gum: An anti-staphylococcal and biocompatible cationic polymer for biotechnological applications', Carbohydrate Polymers, vol. 157, pp. 567-575. https://doi.org/10.1016/j.carbpol.2016.10.026

TY - JOUR

T1 - Quaternized cashew gum

T2 - An anti-staphylococcal and biocompatible cationic polymer for biotechnological applications

AU - Quelemes, Patrick V.

AU - de Araújo, Alyne R.

AU - Plácido, Alexandra

AU - Delerue-Matos, Cristina

AU - Maciel, Jeanny S.

AU - Bessa, Lucinda J.

AU - Ombredane, Alicia S.

AU - Joanitti, Graziella A.

AU - Soares, Maria José dos S.

AU - Eaton, Peter

AU - da Silva, Durcilene A.

AU - Leite, José Roberto S.A.

PY - 2017/2/10

Y1 - 2017/2/10

N2 - Chemical modifications to cashew gum (CG) structure have been previously reported to obtain new physicochemical characteristics, however until now there were no reports of modifications by introduction of new functional groups to add cationic character. This study presents a quaternization route for CG using a quaternary ammonium reagent. The chemical features of the quaternized cashew gum derivatives (QCG) were analyzed by: FTIR, elemental analysis, degree of substitution, Zeta potential, 1H NMR and 1H-13C correlation (HSQC). QCG were evaluated for their anti-staphylococcal activity by determining minimum inhibitory and bactericidal concentrations against pathogenic Staphylococcus spp. and by imaging using atomic force microscopy. Moreover, the mammalian cell biocompatibility were also assessed through hemolytic and cell toxicity assays. QCG presented promising antimicrobial activity against methicillin-resistant S. aureus and biocompatibility on tested cells. These results show that QCG could be a promising tool in the development of biomaterials with an anti-septic action.

AB - Chemical modifications to cashew gum (CG) structure have been previously reported to obtain new physicochemical characteristics, however until now there were no reports of modifications by introduction of new functional groups to add cationic character. This study presents a quaternization route for CG using a quaternary ammonium reagent. The chemical features of the quaternized cashew gum derivatives (QCG) were analyzed by: FTIR, elemental analysis, degree of substitution, Zeta potential, 1H NMR and 1H-13C correlation (HSQC). QCG were evaluated for their anti-staphylococcal activity by determining minimum inhibitory and bactericidal concentrations against pathogenic Staphylococcus spp. and by imaging using atomic force microscopy. Moreover, the mammalian cell biocompatibility were also assessed through hemolytic and cell toxicity assays. QCG presented promising antimicrobial activity against methicillin-resistant S. aureus and biocompatibility on tested cells. These results show that QCG could be a promising tool in the development of biomaterials with an anti-septic action.

KW - Antibacterial

KW - Biocompatibility

KW - Cashew gum

KW - Quaternization

KW - Staphylococcus spp.

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U2 - 10.1016/j.carbpol.2016.10.026

DO - 10.1016/j.carbpol.2016.10.026

M3 - Article

AN - SCOPUS:84992186961

SN - 0144-8617

VL - 157

SP - 567

EP - 575

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

ER -

Quaternized cashew gum: An anti-staphylococcal and biocompatible cationic polymer for biotechnological applications

Abstract

Keywords

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