Site of tubulin synthesis in Tetrahymena pyriformis

Isabel Barahona; Claudina Rodrigues-Pousada

doi:10.1016/0014-5793(83)80593-6

Site of tubulin synthesis in Tetrahymena pyriformis

Isabel Barahona, Claudina Rodrigues-Pousada

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Free and membrane-bound polysomes were isolated from the protozoa Tetrahymena pyriformis, and the contribution of these two types of polysomes to tubulin synthesis were studied using immunoprecipitation of the ³⁵S-translational products in a rabbit reticulocyte lysate. One-dimensional electrophoretic analysis shows that tubulin is synthesized by polyadenylated RNA isolated from free and membrane-bound polysomes. Non-polyadenylated RNAs of free polysomes are also able to direct tubulin synthesis. Two-dimensional electrophoretic analysis using O'Farrell's system confirms these results and also reveals the existence of the α- and β-tubulin subunits.

Original language	English
Pages (from-to)	271-275
Number of pages	5
Journal	FEBS Letters
Volume	158
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(83)80593-6
Publication status	Published - 25 Jul 1983
Externally published	Yes

Keywords

Immunoprecipitation
In vitro protein synthesis
Membrane-bound and free polysomes
Tubulin

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10.1016/0014-5793(83)80593-6

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title = "Site of tubulin synthesis in Tetrahymena pyriformis",

abstract = "Free and membrane-bound polysomes were isolated from the protozoa Tetrahymena pyriformis, and the contribution of these two types of polysomes to tubulin synthesis were studied using immunoprecipitation of the 35S-translational products in a rabbit reticulocyte lysate. One-dimensional electrophoretic analysis shows that tubulin is synthesized by polyadenylated RNA isolated from free and membrane-bound polysomes. Non-polyadenylated RNAs of free polysomes are also able to direct tubulin synthesis. Two-dimensional electrophoretic analysis using O'Farrell's system confirms these results and also reveals the existence of the α- and β-tubulin subunits.",

keywords = "Immunoprecipitation, In vitro protein synthesis, Membrane-bound and free polysomes, Tubulin",

author = "Isabel Barahona and Claudina Rodrigues-Pousada",

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T1 - Site of tubulin synthesis in Tetrahymena pyriformis

AU - Barahona, Isabel

AU - Rodrigues-Pousada, Claudina

PY - 1983/7/25

Y1 - 1983/7/25

N2 - Free and membrane-bound polysomes were isolated from the protozoa Tetrahymena pyriformis, and the contribution of these two types of polysomes to tubulin synthesis were studied using immunoprecipitation of the 35S-translational products in a rabbit reticulocyte lysate. One-dimensional electrophoretic analysis shows that tubulin is synthesized by polyadenylated RNA isolated from free and membrane-bound polysomes. Non-polyadenylated RNAs of free polysomes are also able to direct tubulin synthesis. Two-dimensional electrophoretic analysis using O'Farrell's system confirms these results and also reveals the existence of the α- and β-tubulin subunits.

AB - Free and membrane-bound polysomes were isolated from the protozoa Tetrahymena pyriformis, and the contribution of these two types of polysomes to tubulin synthesis were studied using immunoprecipitation of the 35S-translational products in a rabbit reticulocyte lysate. One-dimensional electrophoretic analysis shows that tubulin is synthesized by polyadenylated RNA isolated from free and membrane-bound polysomes. Non-polyadenylated RNAs of free polysomes are also able to direct tubulin synthesis. Two-dimensional electrophoretic analysis using O'Farrell's system confirms these results and also reveals the existence of the α- and β-tubulin subunits.

KW - Immunoprecipitation

KW - In vitro protein synthesis

KW - Membrane-bound and free polysomes

KW - Tubulin

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DO - 10.1016/0014-5793(83)80593-6

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SN - 0014-5793

VL - 158

SP - 271

EP - 275

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Site of tubulin synthesis in Tetrahymena pyriformis

Abstract

Keywords

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