Specific Insulin‐Mediated Regulation of Glutamine Synthetase in Cultured Chick Astroglial Cells

G. Tholey; A. H. Sena; M. Ledig

doi:10.1111/j.1471-4159.1986.tb00783.x

Specific Insulin‐Mediated Regulation of Glutamine Synthetase in Cultured Chick Astroglial Cells

G. Tholey, A. H. Sena, M. Ledig

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Abstract

Abstract The expression of glutamine synthetase (GS; L‐glutamate ammonia ligase; EC 6.3.1.2) in primary cultures of chick astroglial cells and neurons grown in a chemically defined medium, with and without insulin added, was investigated. An inhibitory effect of insulin toward GS activity, and specific to chick astroglial cells, was observed. Neurons in culture were not sensitive to the hormone effect. Modulation of the activating effect of hydrocortisone onglial GS by insulin was also observed. The data suggest that insulin contributes to the regulation of the metabolism of amino acid neurotransmitters via its effect on GS.

Original language	English
Pages (from-to)	1490-1492
Number of pages	3
Journal	Journal of Neurochemistry
Volume	47
Issue number	5
DOIs	https://doi.org/10.1111/j.1471-4159.1986.tb00783.x
Publication status	Published - Nov 1986
Externally published	Yes

Keywords

Chick astroglial cells
Chick neurons
Glutamine synthetase
Hydro‐cortisone effect
Insulin effect
Primary cultures

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10.1111/j.1471-4159.1986.tb00783.x

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title = "Specific Insulin‐Mediated Regulation of Glutamine Synthetase in Cultured Chick Astroglial Cells",

abstract = "Abstract The expression of glutamine synthetase (GS; L‐glutamate ammonia ligase; EC 6.3.1.2) in primary cultures of chick astroglial cells and neurons grown in a chemically defined medium, with and without insulin added, was investigated. An inhibitory effect of insulin toward GS activity, and specific to chick astroglial cells, was observed. Neurons in culture were not sensitive to the hormone effect. Modulation of the activating effect of hydrocortisone onglial GS by insulin was also observed. The data suggest that insulin contributes to the regulation of the metabolism of amino acid neurotransmitters via its effect on GS.",

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AU - Tholey, G.

AU - Sena, A. H.

AU - Ledig, M.

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N2 - Abstract The expression of glutamine synthetase (GS; L‐glutamate ammonia ligase; EC 6.3.1.2) in primary cultures of chick astroglial cells and neurons grown in a chemically defined medium, with and without insulin added, was investigated. An inhibitory effect of insulin toward GS activity, and specific to chick astroglial cells, was observed. Neurons in culture were not sensitive to the hormone effect. Modulation of the activating effect of hydrocortisone onglial GS by insulin was also observed. The data suggest that insulin contributes to the regulation of the metabolism of amino acid neurotransmitters via its effect on GS.

AB - Abstract The expression of glutamine synthetase (GS; L‐glutamate ammonia ligase; EC 6.3.1.2) in primary cultures of chick astroglial cells and neurons grown in a chemically defined medium, with and without insulin added, was investigated. An inhibitory effect of insulin toward GS activity, and specific to chick astroglial cells, was observed. Neurons in culture were not sensitive to the hormone effect. Modulation of the activating effect of hydrocortisone onglial GS by insulin was also observed. The data suggest that insulin contributes to the regulation of the metabolism of amino acid neurotransmitters via its effect on GS.

KW - Chick astroglial cells

KW - Chick neurons

KW - Glutamine synthetase

KW - Hydro‐cortisone effect

KW - Insulin effect

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Specific Insulin‐Mediated Regulation of Glutamine Synthetase in Cultured Chick Astroglial Cells

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Keywords

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