Star-Shaped Gold Nanoparticles as Friendly Interfaces for Protein Electrochemistry: the Case Study of Cytochrome c

Célia M. Silveira; Rosaceleste Zumpano; Miguel Moreira; Miguel Peixoto de Almeida; Maria João Oliveira; Marina Bento; Cláudia Montez; Inês Paixão; Ricardo Franco; Eulália Pereira; M. Gabriela Almeida

doi:10.1002/celc.201901393

Star-Shaped Gold Nanoparticles as Friendly Interfaces for Protein Electrochemistry: the Case Study of Cytochrome c

Célia M. Silveira, Rosaceleste Zumpano, Miguel Moreira, Miguel Peixoto de Almeida, Maria João Oliveira, Marina Bento, Cláudia Montez, Inês Paixão, Ricardo Franco, Eulália Pereira, M. Gabriela Almeida

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Gold nanostars with an average tip-to-tip length of 52±6 nm were functionalized with different capping agents and used as electrode modification materials for protein electrochemistry. Direct electron transfer between cytochrome c and nanostar-coated pyrolytic graphite electrodes was observed with the protein in solution. The electrochemical response was improved at nanostars functionalized with a 1 : 1 mixture of 11-mercaptoundecanoic acid and 4-mercaptobenzoic acid in comparison with gold nanospheres coated with a similar functionalization. Further immobilization of cytochrome c on pyrolytic graphite while conjugated with the same nanostars guaranteed the maintenance of the protein's native properties, whereas direct adsorption on the bare or nanostar-modified electrodes resulted in an altered conformational state. The pseudo-peroxidase activity of the altered cytochrome c was enhanced in the presence of the nanostars.

Original language	English
Pages (from-to)	4696-4703
Number of pages	8
Journal	ChemElectroChem
Volume	6
Issue number	17
DOIs	https://doi.org/10.1002/celc.201901393
Publication status	Published - 2 Sept 2019

Keywords

cytochrome c
direct electron transfer
gold nanostars
nanoparticles
protein electrochemistry

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10.1002/celc.201901393

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title = "Star-Shaped Gold Nanoparticles as Friendly Interfaces for Protein Electrochemistry: the Case Study of Cytochrome c",

abstract = "Gold nanostars with an average tip-to-tip length of 52±6 nm were functionalized with different capping agents and used as electrode modification materials for protein electrochemistry. Direct electron transfer between cytochrome c and nanostar-coated pyrolytic graphite electrodes was observed with the protein in solution. The electrochemical response was improved at nanostars functionalized with a 1 : 1 mixture of 11-mercaptoundecanoic acid and 4-mercaptobenzoic acid in comparison with gold nanospheres coated with a similar functionalization. Further immobilization of cytochrome c on pyrolytic graphite while conjugated with the same nanostars guaranteed the maintenance of the protein's native properties, whereas direct adsorption on the bare or nanostar-modified electrodes resulted in an altered conformational state. The pseudo-peroxidase activity of the altered cytochrome c was enhanced in the presence of the nanostars.",

keywords = "cytochrome c, direct electron transfer, gold nanostars, nanoparticles, protein electrochemistry",

author = "Silveira, {C{\'e}lia M.} and Rosaceleste Zumpano and Miguel Moreira and {de Almeida}, {Miguel Peixoto} and Oliveira, {Maria Jo{\~a}o} and Marina Bento and Cl{\'a}udia Montez and In{\^e}s Paix{\~a}o and Ricardo Franco and Eul{\'a}lia Pereira and Almeida, {M. Gabriela}",

note = "Publisher Copyright: {\textcopyright} 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2019",

month = sep,

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doi = "10.1002/celc.201901393",

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T1 - Star-Shaped Gold Nanoparticles as Friendly Interfaces for Protein Electrochemistry

T2 - the Case Study of Cytochrome c

AU - Silveira, Célia M.

AU - Zumpano, Rosaceleste

AU - Moreira, Miguel

AU - de Almeida, Miguel Peixoto

AU - Oliveira, Maria João

AU - Bento, Marina

AU - Montez, Cláudia

AU - Paixão, Inês

AU - Franco, Ricardo

AU - Pereira, Eulália

AU - Almeida, M. Gabriela

PY - 2019/9/2

Y1 - 2019/9/2

N2 - Gold nanostars with an average tip-to-tip length of 52±6 nm were functionalized with different capping agents and used as electrode modification materials for protein electrochemistry. Direct electron transfer between cytochrome c and nanostar-coated pyrolytic graphite electrodes was observed with the protein in solution. The electrochemical response was improved at nanostars functionalized with a 1 : 1 mixture of 11-mercaptoundecanoic acid and 4-mercaptobenzoic acid in comparison with gold nanospheres coated with a similar functionalization. Further immobilization of cytochrome c on pyrolytic graphite while conjugated with the same nanostars guaranteed the maintenance of the protein's native properties, whereas direct adsorption on the bare or nanostar-modified electrodes resulted in an altered conformational state. The pseudo-peroxidase activity of the altered cytochrome c was enhanced in the presence of the nanostars.

AB - Gold nanostars with an average tip-to-tip length of 52±6 nm were functionalized with different capping agents and used as electrode modification materials for protein electrochemistry. Direct electron transfer between cytochrome c and nanostar-coated pyrolytic graphite electrodes was observed with the protein in solution. The electrochemical response was improved at nanostars functionalized with a 1 : 1 mixture of 11-mercaptoundecanoic acid and 4-mercaptobenzoic acid in comparison with gold nanospheres coated with a similar functionalization. Further immobilization of cytochrome c on pyrolytic graphite while conjugated with the same nanostars guaranteed the maintenance of the protein's native properties, whereas direct adsorption on the bare or nanostar-modified electrodes resulted in an altered conformational state. The pseudo-peroxidase activity of the altered cytochrome c was enhanced in the presence of the nanostars.

KW - cytochrome c

KW - direct electron transfer

KW - gold nanostars

KW - nanoparticles

KW - protein electrochemistry

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DO - 10.1002/celc.201901393

M3 - Article

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SN - 2196-0216

VL - 6

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JO - ChemElectroChem

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IS - 17

ER -

Star-Shaped Gold Nanoparticles as Friendly Interfaces for Protein Electrochemistry: the Case Study of Cytochrome c

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Keywords

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