TY - JOUR
T1 - Structure and function of a novel antioxidant peptide from the skin of tropical frogs
AU - Barbosa, Eder Alves
AU - Oliveira, Ana
AU - Plácido, Alexandra
AU - Socodato, Renato
AU - Portugal, Camila C.
AU - Mafud, Ana Carolina
AU - Ombredane, Alicia S.
AU - Moreira, Daniel C.
AU - Vale, Nuno
AU - Bessa, Lucinda J.
AU - Joanitti, Graziella A.
AU - Alves, Cláudia
AU - Gomes, Paula
AU - Delerue-Matos, Cristina
AU - Mascarenhas, Yvonne Primerano
AU - Marani, Mariela M.
AU - Relvas, João B.
AU - Pintado, Manuela
AU - Leite, José Roberto S.A.
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2018/2/1
Y1 - 2018/2/1
N2 - The amphibian skin plays an important role protecting the organism from external harmful factors such as microorganisms or UV radiation. Based on biorational strategies, many studies have investigated the cutaneous secretion of anurans as a source of bioactive molecules. By a peptidomic approach, a novel antioxidant peptide (AOP) with in vitro free radical scavenging ability was isolated from Physalaemus nattereri. The AOP, named antioxidin-I, has a molecular weight [M+H] + = 1543.69 Da and a TWYFITPYIPDK primary amino acid sequence. The gene encoding the antioxidin-I precursor was expressed in the skin tissue of three other Tropical frog species: Phyllomedusa tarsius, P. distincta and Pithecopus rohdei. cDNA sequencing revealed highly homologous regions (signal peptide and acidic region). Mature antioxidin-I has a novel primary sequence with low similarity compared with previously described amphibian's AOPs. Antioxidin-I adopts a random structure even at high concentrations of hydrophobic solvent, it has poor antimicrobial activity and poor performance in free radical scavenging assays in vitro, with the exception of the ORAC assay. However, antioxidin-I presented a low cytotoxicity and suppressed menadione-induced redox imbalance when tested with fibroblast in culture. In addition, it had the capacity to substantially attenuate the hypoxia-induced production of reactive oxygen species when tested in hypoxia exposed living microglial cells, suggesting a potential neuroprotective role for this peptide.
AB - The amphibian skin plays an important role protecting the organism from external harmful factors such as microorganisms or UV radiation. Based on biorational strategies, many studies have investigated the cutaneous secretion of anurans as a source of bioactive molecules. By a peptidomic approach, a novel antioxidant peptide (AOP) with in vitro free radical scavenging ability was isolated from Physalaemus nattereri. The AOP, named antioxidin-I, has a molecular weight [M+H] + = 1543.69 Da and a TWYFITPYIPDK primary amino acid sequence. The gene encoding the antioxidin-I precursor was expressed in the skin tissue of three other Tropical frog species: Phyllomedusa tarsius, P. distincta and Pithecopus rohdei. cDNA sequencing revealed highly homologous regions (signal peptide and acidic region). Mature antioxidin-I has a novel primary sequence with low similarity compared with previously described amphibian's AOPs. Antioxidin-I adopts a random structure even at high concentrations of hydrophobic solvent, it has poor antimicrobial activity and poor performance in free radical scavenging assays in vitro, with the exception of the ORAC assay. However, antioxidin-I presented a low cytotoxicity and suppressed menadione-induced redox imbalance when tested with fibroblast in culture. In addition, it had the capacity to substantially attenuate the hypoxia-induced production of reactive oxygen species when tested in hypoxia exposed living microglial cells, suggesting a potential neuroprotective role for this peptide.
KW - Amphibia
KW - Antioxidant peptide
KW - Antioxidin
KW - Microglia
KW - Reactive oxygen species
KW - Skin secretion
UR - http://www.scopus.com/inward/record.url?scp=85034997591&partnerID=8YFLogxK
U2 - 10.1016/j.freeradbiomed.2017.11.001
DO - 10.1016/j.freeradbiomed.2017.11.001
M3 - Article
C2 - 29162516
AN - SCOPUS:85034997591
SN - 0891-5849
VL - 115
SP - 68
EP - 79
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
ER -