Survey of Klebsiella pneumoniae producing extended-spectrum β-lactamases at a Portuguese hospital: TEM-10 as the endemic enzyme

H. Barroso; A. Freitas-Vieira; L. M. Lito; J. Melo Cristino; M. J. Salgado; H. Ferreira Neto; J. C. Sousa; G. Soveral; T. Moura; A. Duarte

doi:10.1093/jac/45.5.611

Survey of Klebsiella pneumoniae producing extended-spectrum β-lactamases at a Portuguese hospital: TEM-10 as the endemic enzyme

H. Barroso, A. Freitas-Vieira, L. M. Lito, J. Melo Cristino, M. J. Salgado, H. Ferreira Neto, J. C. Sousa, G. Soveral, T. Moura, A. Duarte

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34 Citations (Scopus)

Abstract

One hundred and thirty-eight isolates of Klebsiella pneumoniae showing resistance to ceftazidime were isolated from different wards of the Hospital de Santa Maria, Lisbon. The genomic DNA of the isolates was analysed by pulsed-field gel electrophoresis (PFGE) and two patterns were predominant. In all isolates the presence of a single large plasmid of about 50 kb suggested that propagation of the outbreak prominently involved plasmid spread. The deduced amino acid sequence indicated the presence of a TEM-10 β-lactamase. This extended-spectrum β-lactamase was present among K. pneumoniae isolates, was widely disseminated in different wards and remained persistent as a result of an outbreak involving the dissemination of both the multi-resistance plasmids harbouring the bla gene and the isolates themselves.

Original language	English
Pages (from-to)	611-616
Number of pages	6
Journal	Journal of Antimicrobial Chemotherapy
Volume	45
Issue number	5
DOIs	https://doi.org/10.1093/jac/45.5.611
Publication status	Published - 2000

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Barroso, H., Freitas-Vieira, A., Lito, L. M., Melo Cristino, J., Salgado, M. J., Ferreira Neto, H., Sousa, J. C., Soveral, G., Moura, T., & Duarte, A. (2000). Survey of Klebsiella pneumoniae producing extended-spectrum β-lactamases at a Portuguese hospital: TEM-10 as the endemic enzyme. Journal of Antimicrobial Chemotherapy, 45(5), 611-616. https://doi.org/10.1093/jac/45.5.611

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title = "Survey of Klebsiella pneumoniae producing extended-spectrum β-lactamases at a Portuguese hospital: TEM-10 as the endemic enzyme",

abstract = "One hundred and thirty-eight isolates of Klebsiella pneumoniae showing resistance to ceftazidime were isolated from different wards of the Hospital de Santa Maria, Lisbon. The genomic DNA of the isolates was analysed by pulsed-field gel electrophoresis (PFGE) and two patterns were predominant. In all isolates the presence of a single large plasmid of about 50 kb suggested that propagation of the outbreak prominently involved plasmid spread. The deduced amino acid sequence indicated the presence of a TEM-10 β-lactamase. This extended-spectrum β-lactamase was present among K. pneumoniae isolates, was widely disseminated in different wards and remained persistent as a result of an outbreak involving the dissemination of both the multi-resistance plasmids harbouring the bla gene and the isolates themselves.",

author = "H. Barroso and A. Freitas-Vieira and Lito, {L. M.} and {Melo Cristino}, J. and Salgado, {M. J.} and {Ferreira Neto}, H. and Sousa, {J. C.} and G. Soveral and T. Moura and A. Duarte",

note = "Funding Information: Dr. T. Ioroi is a recipient of a grant from the Netherlands Organization for International Cooperation in Higher Education (NUFFIC)",

year = "2000",

doi = "10.1093/jac/45.5.611",

language = "English",

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Barroso, H, Freitas-Vieira, A, Lito, LM, Melo Cristino, J, Salgado, MJ, Ferreira Neto, H, Sousa, JC, Soveral, G, Moura, T & Duarte, A 2000, 'Survey of Klebsiella pneumoniae producing extended-spectrum β-lactamases at a Portuguese hospital: TEM-10 as the endemic enzyme', Journal of Antimicrobial Chemotherapy, vol. 45, no. 5, pp. 611-616. https://doi.org/10.1093/jac/45.5.611

TY - JOUR

T1 - Survey of Klebsiella pneumoniae producing extended-spectrum β-lactamases at a Portuguese hospital

T2 - TEM-10 as the endemic enzyme

AU - Barroso, H.

AU - Freitas-Vieira, A.

AU - Lito, L. M.

AU - Melo Cristino, J.

AU - Salgado, M. J.

AU - Ferreira Neto, H.

AU - Sousa, J. C.

AU - Soveral, G.

AU - Moura, T.

AU - Duarte, A.

N1 - Funding Information: Dr. T. Ioroi is a recipient of a grant from the Netherlands Organization for International Cooperation in Higher Education (NUFFIC)

PY - 2000

Y1 - 2000

N2 - One hundred and thirty-eight isolates of Klebsiella pneumoniae showing resistance to ceftazidime were isolated from different wards of the Hospital de Santa Maria, Lisbon. The genomic DNA of the isolates was analysed by pulsed-field gel electrophoresis (PFGE) and two patterns were predominant. In all isolates the presence of a single large plasmid of about 50 kb suggested that propagation of the outbreak prominently involved plasmid spread. The deduced amino acid sequence indicated the presence of a TEM-10 β-lactamase. This extended-spectrum β-lactamase was present among K. pneumoniae isolates, was widely disseminated in different wards and remained persistent as a result of an outbreak involving the dissemination of both the multi-resistance plasmids harbouring the bla gene and the isolates themselves.

AB - One hundred and thirty-eight isolates of Klebsiella pneumoniae showing resistance to ceftazidime were isolated from different wards of the Hospital de Santa Maria, Lisbon. The genomic DNA of the isolates was analysed by pulsed-field gel electrophoresis (PFGE) and two patterns were predominant. In all isolates the presence of a single large plasmid of about 50 kb suggested that propagation of the outbreak prominently involved plasmid spread. The deduced amino acid sequence indicated the presence of a TEM-10 β-lactamase. This extended-spectrum β-lactamase was present among K. pneumoniae isolates, was widely disseminated in different wards and remained persistent as a result of an outbreak involving the dissemination of both the multi-resistance plasmids harbouring the bla gene and the isolates themselves.

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DO - 10.1093/jac/45.5.611

M3 - Article

AN - SCOPUS:0034015848

SN - 0305-7453

VL - 45

SP - 611

EP - 616

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 5

ER -

Survey of Klebsiella pneumoniae producing extended-spectrum β-lactamases at a Portuguese hospital: TEM-10 as the endemic enzyme

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