TY - JOUR
T1 - Synthesis of WO3 nanoparticles for biosensing applications
AU - Santos, Lídia
AU - Silveira, Célia M.
AU - Elangovan, Elamurugu
AU - Neto, Joana P.
AU - Nunes, Daniela
AU - Pereira, Luís
AU - Martins, Rodrigo
AU - Viegas, Jaime
AU - Moura, José J.G.
AU - Todorovic, Smilja
AU - Almeida, M. Gabriela
AU - Fortunato, Elvira
N1 - Publisher Copyright:
© 2015 Elsevier B.V. All rights reserved.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Direct electron transfer with redox proteins, in third generation biosensors, is already proved to be favored on electrodes modified with nanoparticles. In this work, different crystallographic and morphologic structures of tungsten oxide (WO3) nanoparticles are modified by hydrothermal synthesis at 180°C. The electrochemical properties of WO3 nanoparticles deposit on ITO electrodes are investigated and the analytical performance of the nitrite biosensor is presented as proof of concept. Despite the inherent features of each nanostructure, the heterogeneous electron transfer with the WO3 nanoparticles modified electrodes is thoroughly improved and, very importantly, the cytochrome c nitrite reductase (ccNiR) enzyme is able to keep its biological function. When compared with bare commercial ITO electrodes, the exchange rate constant of WO3/ITO electrodes with cytochrome c increased one order of magnitude, while the analytical parameters of the ccNiR/WO3/ITO electrodes response to nitrite (the Michaelis-Menten constant is 47 μM and sensitivity of 2143 mA M-1 cm-2) are comparable to those reported for carbon based electrodes. Therefore, these metal oxide nanoparticles are good alternative materials for electrochemical applications, such as non-mediated biosensors.
AB - Direct electron transfer with redox proteins, in third generation biosensors, is already proved to be favored on electrodes modified with nanoparticles. In this work, different crystallographic and morphologic structures of tungsten oxide (WO3) nanoparticles are modified by hydrothermal synthesis at 180°C. The electrochemical properties of WO3 nanoparticles deposit on ITO electrodes are investigated and the analytical performance of the nitrite biosensor is presented as proof of concept. Despite the inherent features of each nanostructure, the heterogeneous electron transfer with the WO3 nanoparticles modified electrodes is thoroughly improved and, very importantly, the cytochrome c nitrite reductase (ccNiR) enzyme is able to keep its biological function. When compared with bare commercial ITO electrodes, the exchange rate constant of WO3/ITO electrodes with cytochrome c increased one order of magnitude, while the analytical parameters of the ccNiR/WO3/ITO electrodes response to nitrite (the Michaelis-Menten constant is 47 μM and sensitivity of 2143 mA M-1 cm-2) are comparable to those reported for carbon based electrodes. Therefore, these metal oxide nanoparticles are good alternative materials for electrochemical applications, such as non-mediated biosensors.
KW - Biosensor
KW - Electrochemistry
KW - Hydrothermal synthesis
KW - Nanostructures
KW - Tungsten oxide
UR - http://www.scopus.com/inward/record.url?scp=84943150062&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2015.09.046
DO - 10.1016/j.snb.2015.09.046
M3 - Article
AN - SCOPUS:84943150062
SN - 0925-4005
VL - 223
SP - 186
EP - 194
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -