The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing

Manuel M. Vicente; Miguel Chaves-Ferreira; João M.P. Jorge; João T. Proença; Vasco M. Barreto

doi:10.3389/fcell.2021.718466

The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing

Manuel M. Vicente, Miguel Chaves-Ferreira, João M.P. Jorge, João T. Proença, Vasco M. Barreto

Research output: Contribution to journal › Review article › peer-review

15 Citations (Scopus)

Abstract

The repurposing of the CRISPR/Cas bacterial defense system against bacteriophages as simple and flexible molecular tools has revolutionized the field of gene editing. These tools are now widely used in basic research and clinical trials involving human somatic cells. However, a global moratorium on all clinical uses of human germline editing has been proposed because the technology still lacks the required efficacy and safety. Here we focus on the approaches developed since 2013 to decrease the frequency of unwanted mutations (the off-targets) during CRISPR-based gene editing.

Original language	English
Article number	718466
Journal	Frontiers in Cell and Developmental Biology
Volume	9
DOIs	https://doi.org/10.3389/fcell.2021.718466
Publication status	Published - 17 Sept 2021
Externally published	Yes

Keywords

CRISPR/Cas9
Cas9 variants
DNA repair
gene editing
off-targets

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10.3389/fcell.2021.718466

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abstract = "The repurposing of the CRISPR/Cas bacterial defense system against bacteriophages as simple and flexible molecular tools has revolutionized the field of gene editing. These tools are now widely used in basic research and clinical trials involving human somatic cells. However, a global moratorium on all clinical uses of human germline editing has been proposed because the technology still lacks the required efficacy and safety. Here we focus on the approaches developed since 2013 to decrease the frequency of unwanted mutations (the off-targets) during CRISPR-based gene editing.",

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AU - Proença, João T.

AU - Barreto, Vasco M.

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AB - The repurposing of the CRISPR/Cas bacterial defense system against bacteriophages as simple and flexible molecular tools has revolutionized the field of gene editing. These tools are now widely used in basic research and clinical trials involving human somatic cells. However, a global moratorium on all clinical uses of human germline editing has been proposed because the technology still lacks the required efficacy and safety. Here we focus on the approaches developed since 2013 to decrease the frequency of unwanted mutations (the off-targets) during CRISPR-based gene editing.

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KW - DNA repair

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The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing

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Keywords

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