TY - JOUR
T1 - The phytochemical and bioactivity profiles of wild Calluna vulgaris L. flowers
AU - Rodrigues, Francisca
AU - Moreira, Teresa
AU - Pinto, Diana
AU - Pimentel, Filipa B.
AU - Costa, Anabela S.G.
AU - Nunes, M. Antónia
AU - Gonçalves Albuquerque, Tânia
AU - S. Costaa, Helena
AU - Palmeira-de-Oliveira, Ana
AU - Oliveira, Ana I.
AU - Sut, Stefania
AU - Dall'Acqua, Stefano
AU - Oliveira, M. Beatriz P.P.
N1 - Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/9
Y1 - 2018/9
N2 - The nutritional composition of Calluna vulgaris flowers as well as the phytochemical profile, antioxidant (DPPH and FRAP assays), antimicrobial and cytotoxic (in human immortalized non-tumorigenic keratinocyte and fibroblasts) activities of aqueous, hydroalcoholic and ethanolic extracts were evaluated. A high content of fiber and carbohydrates (75%) and the prevalence of α-tocopherol as vitamer deserves attention. Linolenic (35%), linoleic (27%) and palmitic (21%) acids were the most abundant fatty acids. Qualitative and quantitative analysis by LC-MS and 1NMR indicated high levels of quercetin, kaempferol and myricetin derivatives as well as procyanidins. The hydro-alcoholic extract displayed the highest antioxidant activity and total phenolics (TPC) and flavonoid contents (TFC). No adverse effects on cells were observed until a concentration of 100 μg/mL and a good antimicrobial activity was reported against S. epidermidis and S. aureus with the hydro-alcoholic extract. The data obtained demonstrated that wild plants like heather, although not being a common nutritional reference, can be used in an alimentary base as a source of bioactive compounds, namely antioxidants.
AB - The nutritional composition of Calluna vulgaris flowers as well as the phytochemical profile, antioxidant (DPPH and FRAP assays), antimicrobial and cytotoxic (in human immortalized non-tumorigenic keratinocyte and fibroblasts) activities of aqueous, hydroalcoholic and ethanolic extracts were evaluated. A high content of fiber and carbohydrates (75%) and the prevalence of α-tocopherol as vitamer deserves attention. Linolenic (35%), linoleic (27%) and palmitic (21%) acids were the most abundant fatty acids. Qualitative and quantitative analysis by LC-MS and 1NMR indicated high levels of quercetin, kaempferol and myricetin derivatives as well as procyanidins. The hydro-alcoholic extract displayed the highest antioxidant activity and total phenolics (TPC) and flavonoid contents (TFC). No adverse effects on cells were observed until a concentration of 100 μg/mL and a good antimicrobial activity was reported against S. epidermidis and S. aureus with the hydro-alcoholic extract. The data obtained demonstrated that wild plants like heather, although not being a common nutritional reference, can be used in an alimentary base as a source of bioactive compounds, namely antioxidants.
KW - Bioactivity
KW - Calluna vulgaris (L.) hull
KW - Natural antioxidants
KW - Phenolic compounds
UR - http://www.scopus.com/inward/record.url?scp=85048289556&partnerID=8YFLogxK
U2 - 10.1016/j.foodres.2018.06.012
DO - 10.1016/j.foodres.2018.06.012
M3 - Article
C2 - 30007738
AN - SCOPUS:85048289556
SN - 0963-9969
VL - 111
SP - 724
EP - 731
JO - Food Research International
JF - Food Research International
ER -