TY - GEN
T1 - The value of a new cancer biomarker fHER-2 proto-oncogene in the diagnosis of feline mammary carcinoma
AU - Soares, Maria
AU - Correia, Jorge
AU - Cabeçadas, José
AU - Peleteiro, Conceição
AU - Ferreira, Fernando
PY - 2012
Y1 - 2012
N2 - The overexpression of the Human Epidermal growth factor Receptor-2 (HER-2) oncogene in human breast cancer is associated with a poor prognosis and a specific treatment. Because of its importance and as a first line option for diagnosis, well established guidelines for its detection are based in immunohistochemical techniques, Still, in Veterinary Medicine there is little and inconsistent information about this subject. The aim of our study was to achieve an optimal immunohistochemical protocol for detection of fHER-2 in Feline Mammary Carcinoma (FMC). Five commercial anti-HER-2 antibodies were tested using three different protocols. The fHER-2 protein overexpression was detected in 10 of the 30 FMC cases (33.3%), when the optimized protocol was performed (associating the A0485 antibody with a longer antigen retrieval method). These results suggest that fHER-2 may play an important role in Feline Oncology and that the Cat can be a suitable animal model for human breast cancer research.
AB - The overexpression of the Human Epidermal growth factor Receptor-2 (HER-2) oncogene in human breast cancer is associated with a poor prognosis and a specific treatment. Because of its importance and as a first line option for diagnosis, well established guidelines for its detection are based in immunohistochemical techniques, Still, in Veterinary Medicine there is little and inconsistent information about this subject. The aim of our study was to achieve an optimal immunohistochemical protocol for detection of fHER-2 in Feline Mammary Carcinoma (FMC). Five commercial anti-HER-2 antibodies were tested using three different protocols. The fHER-2 protein overexpression was detected in 10 of the 30 FMC cases (33.3%), when the optimized protocol was performed (associating the A0485 antibody with a longer antigen retrieval method). These results suggest that fHER-2 may play an important role in Feline Oncology and that the Cat can be a suitable animal model for human breast cancer research.
UR - http://www.scopus.com/inward/record.url?scp=84861972925&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84861972925
SN - 9789898425942
T3 - Proceedings of the International Workshop on Veterinary Biosignals and Biodevices, VBB 2012, in Conjunction with BIOSTEC 2012
SP - 9
EP - 17
BT - Proceedings of the International Workshop on Veterinary Biosignals and Biodevices, VBB 2012, in Conjunction with BIOSTEC 2012
T2 - 1st International Workshop on Veterinary Biosignals and Biodevices, VBB 2012, in Conjunction with BIOSTEC 2012
Y2 - 1 February 2012 through 4 February 2012
ER -
